Human Leukotriene B4 R1 PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
  • Specificity
    Recognizes human Leukotriene B4 R1 (5).
  • Source
    Monoclonal Mouse IgG1 Clone # 203/14F11
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 PE‑conjugated Monoclonal Antibody (Catalog # FAB099P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Leukotriene B4 R1
Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9). Enhanced LTB4 production and engagement of the BLT receptors can be important in allergic and inflammatory diseases such as asthma (10), allergic encephalomyelitis (11), endotoxic shock (12), ischemia (12), psoriasis (13), rheumatoid arthritis (14) and inflammatory bowel disease (15). In addition, it has been reported that BLT1 can function as an additional co-receptor for HIV infection of CD4+ T cells (16, 17). Investigations into the mechanisms and potential inhibitors of LTB4 binding to its’ receptors may provide insight into possible treatment modalities for a number of inflammatory disorders.
  • References:
    1. Samuelsson, B. et al. (1987) Science 237:1171.
    2. Brom, J. and W. Konig (1989) Immunology 68:479.
    3. Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
    4. Dasari, V.R. et al. (2000) Immunopharm. 48:157.
    5. Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
    6. Yokomizo, T. et al. (1997) Nature 387:620.
    7. Kato, K. et al. (2000) J. Exp. Med. 192:413.
    8. Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
    9. Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.
    10. Turner, C.R. et al. (1996) J. Clin. Invest. 97:381.
    11. Gladue, R.P. et al. (1996) J. Exp. Med. 183:1893.
    12. Lefer, A.M. (1986) Biochem. Pharmacol. 35:123.
    13. Iverson, L. et al. (1997) Skin Pharmacol. 10:169.
    14. Griffiths, R.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:517.
    15. Sharon, P. and W.F. Stenson (1984) Gastroenterology 86:453.
    16. Owman, C. et al. (1996) Proc. Natl. Acad. Sci. USA 95:9530.
    17. Martin, V. et al. (1999) J. Biol. Chem. 274:8597.
  • Long Name:
    Leukotriene B4 Receptor 1
  • Entrez Gene IDs:
    1241 (Human)
  • Alternate Names:
    BLT1; chemokine receptor-like 1; CMKRL1; leukotriene B4 receptor; Leukotriene B4R1; LTB4R; LTB4R1; LTBR1; P2Y purinoceptor 7; purinergic receptor P2Y, G-protein coupled, 7
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Expression of enzymes and receptors of the leukotriene pathway in human neuroblastoma promotes tumor survival and provides a target for therapy.
    Authors: Sveinbjornsson B, Rasmuson A, Baryawno N, Wan M, Pettersen I, Ponthan F, Orrego A, Haeggstrom JZ, Johnsen JI, Kogner P
    FASEB J., 2008;22(10):3525-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Residues from transmembrane helices 3 and 5 participate in leukotriene B4 binding to BLT1.
    Authors: Sabirsh A, Bywater RP, Bristulf J, Owman C, Haeggstrom JZ
    Biochemistry, 2006;45(18):5733-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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