Detection of Leukotriene B4 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Leukotriene B4 R1 PE‑conjugated Monoclonal Antibody (Catalog # FAB099P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Leukotriene B4 R1
Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9). Enhanced LTB4 production and engagement of the BLT receptors can be important in allergic and inflammatory diseases such as asthma (10), allergic encephalomyelitis (11), endotoxic shock (12), ischemia (12), psoriasis (13), rheumatoid arthritis (14) and inflammatory bowel disease (15). In addition, it has been reported that BLT1 can function as an additional co-receptor for HIV infection of CD4+ T cells (16, 17). Investigations into the mechanisms and potential inhibitors of LTB4 binding to its’ receptors may provide insight into possible treatment modalities for a number of inflammatory disorders.
Samuelsson, B. et al. (1987) Science 237:1171.
Brom, J. and W. Konig (1989) Immunology 68:479.
Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
Dasari, V.R. et al. (2000) Immunopharm. 48:157.
Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
Yokomizo, T. et al. (1997) Nature 387:620.
Kato, K. et al. (2000) J. Exp. Med. 192:413.
Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.
Turner, C.R. et al. (1996) J. Clin. Invest. 97:381.
Gladue, R.P. et al. (1996) J. Exp. Med. 183:1893.
Lefer, A.M. (1986) Biochem. Pharmacol. 35:123.
Iverson, L. et al. (1997) Skin Pharmacol. 10:169.
Griffiths, R.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:517.
Sharon, P. and W.F. Stenson (1984) Gastroenterology 86:453.
Owman, C. et al. (1996) Proc. Natl. Acad. Sci. USA 95:9530.
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