Human LIF Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse LIF, recombinant human (rh) CLC, rhCNTF, rhCardiotrophin-1, rhIL-6, rhIL-11, or rhOSM is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 9808
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human LIF
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of LIF in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LIF Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC2501R, filled histogram) or isotype control antibody(Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LIF
LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells.
  • References:
    1. Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337.
    2. Gough, N.M. (1992) Growth Factors 7:175.
    3. Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
    4. Gillett, N.A. et al. (1993) Growth Factors 9:301.
    5. Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
    6. Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
    7. Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
    8. Williams, R.L. et al. (1988) Nature 336:684.
    9. Baumann, H. et al. (1989) J. Immunol. 143:1163.
    10. Marshall, M.K. et al. (1994) Endocrinology 135:1412.
    11. Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
    12. Davis, S. et al. (1993) Science 260:18054.
    13. Gearing, D.P. et al. (1991) EMBO J. 10:28395.
    14. Gearing, D.P. et al. (1991) Science 255:1434.
    15. Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
    16. Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.
  • Long Name:
    Leukemia Inhibitory Factor
  • Entrez Gene IDs:
    3976 (Human); 16878 (Mouse); 403449 (Canine)
  • Alternate Names:
    CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI
Related Research Areas

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