Detects human LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse LIF, recombinant human (rh) CLC, rhCNTF, rhCardiotrophin-1, rhIL-6, rhIL-11, or rhOSM is observed.
Monoclonal Mouse IgG1 Clone # 9808
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human LIF
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Detection of LIF in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LIF Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC2501R, filled histogram) or isotype control antibody(Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a
variety of cells including T cells (3), monocytes (4), fibroblasts (5),
osteoblasts (6) and mast cells (7). Consistent with its many synonyms,
LIF exhibits a broad spectrum of effects on both hematopoietic and
nonhematopoietic cells. For example, LIF inhibits the differentiation of
embryonic stem cells (8), up regulates the synthesis of acute phase
proteins in hepatocytes (9), down regulates lipoprotein lipase activity
in adipocytes (10), and preferentially induces a cholinergic phenotype
in sympathetic neurons (11). The receptor for LIF (LIF R) has been
isolated and found to be a 190 kDa type I transmembrane glycoprotein
(12). Although this molecule binds LIF, the resultant LIF-LIF R complex
is not sufficient to transduce an intracellular signal. This capability
is provided by a 130 kDa signal transducing subunit (gp130) that is
common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin
M (13, 14). Since gp130 is a ubiquitously expressed membrane protein,
the presence of LIF R (membrane-bound or soluble form) ultimately
determines the cell’s responsiveness to LIF. Cells known to express LIF R
include osteoblasts (6), hepatocytes (15), macrophages (15), neurons
(5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence
homology, and human LIF is biologically active on mouse cells.
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Gough, N.M. (1992) Growth Factors 7:175.
Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
Gillett, N.A. et al. (1993) Growth Factors 9:301.
Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
Williams, R.L. et al. (1988) Nature 336:684.
Baumann, H. et al. (1989) J. Immunol. 143:1163.
Marshall, M.K. et al. (1994) Endocrinology 135:1412.
Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
Davis, S. et al. (1993) Science 260:18054.
Gearing, D.P. et al. (1991) EMBO J. 10:28395.
Gearing, D.P. et al. (1991) Science 255:1434.
Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.
Have you used Human LIF Alexa Fluor® 647-conjugated Antibody?
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