< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
No significant interference observed with available related molecules.
The Quantikine Human LIF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure soluble human LIF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human LIF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant LIF accurately. Results obtained measuring natural human LIF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human LIF.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Cell Culture Supernates
The recovery of LIF spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Media (n=4)
EDTA Plasma (n=2)
Heparin Plasma (n=8)
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex.
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
50 µL Assay Diluent for Serum & Plasma Samples Only
For Serum & Plasma Samples Only: Add 50 µL of Assay Diluent to each well.
200 µL Standard, Control, or Sample
Add 200 µL of Standard, control, or sample to each well.
For Serum & Plasma Samples: Cover with a plate sealer, and incubate at 37 °C for 2 hours. For Cell Culture Supernate Samples: Cover with a plate sealer, and incubate at room temperature for 2 hours.
Aspirate each well and wash, repeating the process twice for a total of 3 washes.
200 µL Conjugate
Add 200 µL of Conjugate to each well.
For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours. For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
Aspirate and wash 3 times.
200 µL Substrate Solution
Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
50 µL Stop Solution
Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.