Human LIN-28B Antibody

Catalog # Availability Size / Price Qty
AF7726
AF7726-SP
Detection of Human LIN-28B by Western Blot.
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Human LIN-28B Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIN-28B in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human LIN-28A is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LIN-28B
Val83-Thr250
Accession # Q6ZN17
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human LIN-28B by Western Blot. View Larger

Detection of Human LIN-28B by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LIN-28B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7726) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LIN-28B at approximately 22 and 35‑37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry LIN-28B in Human Liver. View Larger

LIN-28B in Human Liver. LIN-28B was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human LIN-28B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7726) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIN-28B

Lin-28B (Lineage protein 28 homolog B) is a 35-37 kDa member of the Lin28 family of proteins. Although there is an accompanying Lin-28/28A molecule, Lin-28 and Lin-28B are not alternative splice forms but represent products of separate genes. Lin-28B is found in both the cytoplasm and nucleus of fetal hematopoietic stem and progenitor cells, embryonic liver and multiple tumor types. It is considered a regulator of miRNA activity. Active miRNAs arise from transcribed RNAs that are first cleaved by nuclear RNase III, next cleaved by cytoplasmic Dicer, and finally associate with Argonaute to generate a RISC RNA silencing complex. Lin-28A participates in the cytoplasmic uridylation of miRNAs on their 3’-end, blocking Dicer processing and initiating RNA degradation. Lin-28B, by contrast, acts in the nucleus and blocks the initial RNase III cleavage step. Human Lin-28B is 250 amino acids (aa) in length (SwissProt # Q6ZN17). It contains one CSD/cold shock domain (aa 29-102) plus two CCHC-type Zn-finger RNA bindingdomains (aa 127-144 and 149-166). Phosphorylation is known to occur on Ser105. There is one reported alternate start site at Met71, and there is a potential 11 aa substitution for aa 1-4. Two isoforms of LIN28B with apparent MWs of 22 kDa and 37 kDa have been reported. Over aa 83-250, human Lin-28B shares 84% aa sequence identity with mouse Lin-28B.

Long Name
LIN-28 Homolog B
Entrez Gene IDs
389421 (Human); 380669 (Mouse); 689054 (Rat)
Alternate Names
CSDD2; CSDD2protein lin-28 homolog B; FLJ16517; lin-28 homolog B (C. elegans); Lin-28.2; LIN28B; LIN-28B

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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