Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all LIN-41 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LIN-41
Val386-Phe868
Accession # Q2Q1W2

Specificity

Detects human LIN-41 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LIN‑41 Antibody

Detection of Human LIN‑41 antibody by Western Blot.

Detection of Human LIN‑41 by Western Blot.

Western blot shows lysates of BG0IV human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LIN-41 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

LIN-41 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

LIN‑41 in BG01V Human Stem Cells.

LIN-41 was detected in immersion fixed BG01V human embryonic stem cells using Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human LIN-41 antibody by Simple WesternTM.

Detection of Human LIN‑41 by Simple WesternTM.

Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for LIN-41 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human LIN-41 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5104) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human LIN‑41 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Simple Western

10 µg/mL
Sample: BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: BG0IV human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIN-41

LIN-41 (lineage-41; also TRIM/tripartite motif-containing protein 71) is a 94 kDa (predicted) cytosolic member of the RBCC (RING finger, B-Box Coiled-Coil) family of proteins. It is expressed in developing limbs, DRG and branchial arches and apparently regulates the timed expression of proliferation and differentiation-associated genes. Human LIN-41 is 868 amino acids (aa) in length. It contains a RING-type zinc finger region (aa 12-95), a His-rich segment (aa 146‑157), two B Box zinc fingers that bind DNA (aa 194-314), a coiled-coil motif that binds protein (aa 391-427), one filamin repeat and six NHL repeats that regulate gene translation (aa 593‑868). Over aa 386-868, human LIN-41 is 97%, 95%, and 94% aa identical to canine, mouse, and chicken LIN-41, respectively.

Long Name

RNA-binding Protein LIN-41

Alternate Names

LIN41, TRIM71

Entrez Gene IDs

131405 (Human); 636931 (Mouse); 301042 (Rat)

Gene Symbol

TRIM71

UniProt

Q2Q1W2

Additional LIN-41 Products

Product Documents for Human LIN‑41 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LIN‑41 Antibody

For research use only

Citations for Human LIN‑41 Antibody

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Protocols

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