Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Biotin

Antibody Source

Polyclonal Goat IgG
Loading...

Product Specifications

Immunogen

E. coli-derived recombinant human LMO2 isoform 1
Met1-Ile158
Accession # P25791

Specificity

Detects human LMO2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LMO2 Biotinylated Antibody

LMO2 antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

LMO2 in K562 Human Cell Line.

LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human LMO2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2726) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human LMO2 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human LMO2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.


Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LMO2

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Entrez Gene IDs

4005 (Human)

Gene Symbol

LMO2

UniProt

Additional LMO2 Products

Product Documents for Human LMO2 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LMO2 Biotinylated Antibody

For research use only

Related Research Areas

Customer Reviews for Human LMO2 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human LMO2 Biotinylated Antibody and earn rewards!

Have you used Human LMO2 Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies