Human LMO2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
LMO2 in K562 Human Cell Line.
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Product Details
Supplemental Products

Human LMO2 Biotinylated Antibody Summary

Species Reactivity
Detects human LMO2 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human LMO2 isoform 1
Accession # P25791
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human LMO2
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry LMO2 antibody in K562 Human Cell Line by Immunocytochemistry (ICC). View Larger

LMO2 in K562 Human Cell Line. LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human LMO2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2726) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMO2

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Long Name
LIM Domain Only 2
Entrez Gene IDs
4005 (Human)
Alternate Names
Cysteine-rich protein TTG-2; LIM domain only 2 (rhombotin-like 1); LIM domain only protein 2; LMO2; LMO-2; RBTN2; RBTN2rhombotin-2; RBTNL1; RBTNL1rhombotin-like 1; RHOM2; RHOM2T-cell translocation protein 2; T-cell translocation gene 2; TTG2; TTG2rhombotin 2

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