Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human LMO2
Ser2-Ile158
Accession # P25791

Specificity

Detects human LMO2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LMO2 Antibody

Detection of LMO2 antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of LMO2 in K562 Human Cell Line by Flow Cytometry.

K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.

Detection of LMO2 antibody in Human PBMC antibody by Flow Cytometry

Detection of LMO2 in Human PBMC by Flow Cytometry

Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.

LMO2 antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

LMO2 in K562 Human Cell Line.

LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse LMO2 by Western Blot

Detection of Mouse LMO2 by Western Blot

Gene expression analysis of Tal1−/− Flk-1+ cells. (A) Lmo2, Tal1 gene expression relative to Hprt in Flk-1+ cells derived from WT, Lmo2−/− or Tal1−/− ES cells. Data represent the mean of three independent samples measured in duplicate ± standard deviation. (B) LMO2, TAL1, LDB1 and beta -actin protein levels detected by western blotting using nuclear extract from WT, Lmo2−/− and Tal1−/− Flk-1+ cells. (C) Pairwise comparison of significantly differentially expressed genes. Quantitation and analyses of RNAseq data are based on biological triplicate samples. (D) Heat map showing hierarchical clustering of differentially expressed genes at the Flk-1+ stage. (E) Gene ontology enrichment analysis for biological process was performed on cluster 5 as identified in D. Terms were ordered according to their Modified Fisher Extract P-value and only terms with P < 0.05 were considered significant. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/17/9874/3902958), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LMO2 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line and human PBMC

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human LMO2

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LMO2

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Long Name

LIM Domain Only 2

Alternate Names

RBTN2, RBTNL1, RHOM2, TTG2

Entrez Gene IDs

4005 (Human)

Gene Symbol

LMO2

UniProt

Additional LMO2 Products

Product Documents for Human LMO2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LMO2 Antibody

For research use only

Related Research Areas

Citations for Human LMO2 Antibody

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Protocols

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