Human LPAR2 Antibody

Catalog # Availability Size / Price Qty
AF6396
AF6396-SP
Human LPAR2 Antibody in Western Blot
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Human LPAR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LPAR2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LPAR1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LPAR2
Met1-Val33, His91-Gly103, Ser168-Ser188, Asp264-Tyr279
Accession # Q9HBW0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LPAR2 by Western Blot. View Larger

Detection of Human LPAR2 by Western Blot. Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human LPAR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LPAR2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPAR2/LPA2/EDG-4

LPAR2 (Lysophosphatidic acid receptor 2; also LPA2 and EDG4) is a 39 kDa member of the GPCR1 family of proteins.  It has limited expression, and is found on mammalian macrophages, fibroblasts and cardiac myocytes.  LPAR2 binds to, and is activated by LPA, a molecule principally derived from activated platelets.  Although LPAR2 appears to prefer myristoyl and palmitoyl LPA, multiple forms of LPA are recognized by the receptor.  LPAR2 reportedly mediates chemotaxis, arachidonic acid release, and cell rounding.  Human LPAR2 is a 351 amino acid (aa) 7–transmembrane (TM) protein (SwissProt # Q9HBW0).  It contains a 33 aa extracellular N-terminus (aa #1-33), seven TM segments (aa #34-297) and a 54 aa C-terminal cytoplasmic tail.  There is one splice variant that is associated with tumor cells, and which originally was given the designation EDG4 (endothelium differentiation gene 4).  This shows a 35 aa substitution for aa #348-351.  Over aa #1-33, 91-103, 168-188 and 264-279 collectively, human LPAR2 shares 75% aa identity with mouse LPAR2.  

Long Name
Lysophosphatidic Acid Receptor 2
Entrez Gene IDs
9170 (Human); 53978 (Mouse); 498609 (Rat)
Alternate Names
EDG4; EDG-4; EDG4FLJ93869; endothelial differentiation, lysophosphatidic acid G-protein-coupled receptor4,LPA receptor EDG4; G protein-coupled receptor; LPA2; LPA-2; LPA2LPA receptor 2; LPAR2; lysophosphatidic acid receptor 2; lysophosphatidic acid receptor EDG4; Lysophosphatidic acid receptor Edg-4

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