Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all LPAR2/LPA2/EDG-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LPAR2
Met1-Val33, His91-Gly103, Ser168-Ser188, Asp264-Tyr279
Accession # Q9HBW0

Specificity

Detects human LPAR2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human LPAR1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LPAR2 Antibody

Detection of Human LPAR2 antibody by Western Blot.

Detection of Human LPAR2 by Western Blot.

Western blot shows lysates of human peripheral blood lymphocytes (PBL). PVDF Membrane was probed with 1 µg/mL of Human LPAR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6396) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LPAR2 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human LPAR2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human peripheral blood lymphocytes (PBL)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LPAR2/LPA2/EDG-4

LPAR2 (Lysophosphatidic acid receptor 2; also LPA2 and EDG4) is a 39 kDa member of the GPCR1 family of proteins.  It has limited expression, and is found on mammalian macrophages, fibroblasts and cardiac myocytes.  LPAR2 binds to, and is activated by LPA, a molecule principally derived from activated platelets.  Although LPAR2 appears to prefer myristoyl and palmitoyl LPA, multiple forms of LPA are recognized by the receptor.  LPAR2 reportedly mediates chemotaxis, arachidonic acid release, and cell rounding.  Human LPAR2 is a 351 amino acid (aa) 7–transmembrane (TM) protein (SwissProt # Q9HBW0).  It contains a 33 aa extracellular N-terminus (aa #1-33), seven TM segments (aa #34-297) and a 54 aa C-terminal cytoplasmic tail.  There is one splice variant that is associated with tumor cells, and which originally was given the designation EDG4 (endothelium differentiation gene 4).  This shows a 35 aa substitution for aa #348-351.  Over aa #1-33, 91-103, 168-188 and 264-279 collectively, human LPAR2 shares 75% aa identity with mouse LPAR2.  

Long Name

Lysophosphatidic Acid Receptor 2

Alternate Names

EDG4, LPA2, LPAR2

Entrez Gene IDs

9170 (Human); 53978 (Mouse); 498609 (Rat)

Gene Symbol

LPAR2

UniProt

Q9HBW0 (Human)

Additional LPAR2/LPA2/EDG-4 Products

Product Documents for Human LPAR2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LPAR2 Antibody

For research use only

Citations for Human LPAR2 Antibody

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