Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 990310
View all LRIG2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LRIG-2
Gly41-Thr805
Accession # O94898

Specificity

Detects human LRIG-2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LRIG2 Antibody

Detection of LRIG-2 antibody in HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP antibody by Flow Cytometry.

Detection of LRIG-2 in HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human LRIG-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LRIG-2 Monoclonal Antibody (Catalog # MAB1941) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

LRIG2 antibody in WM-115 Human Cell Line by Immunocytochemistry (ICC).

LRIG2 in WM‑115 Human Cell Line.

LRIG2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB1941) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LRIG2 antibody in IMCD3 Mouse Cell Line by Immunocytochemistry (ICC).

LRIG2 in IMCD3 Mouse Cell Line.

LRIG2 was detected in immersion fixed IMCD3 mouse inner medullary collecting duct cell line using Mouse Anti-Human LRIG2 Monoclonal Antibody (Catalog # MAB1941) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LRIG2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human LRIG-2 and eGFP

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed WM-115 human malignant melanoma cell line and IMCD3 mouse inner medullary collecting duct cell line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRIG2

LRIG2 (leucine-rich repeats and Ig-like domains-2; also LIG-2) is an approximately 119-132 kDa glycoprotein that belongs to the LRIG gene family. The LRIG2 protein was predicted to have the same domain organization as LRIG1with a signal peptide, an extracellular part containing 15 leucine-rich repeats and three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. Over aa 41‑805, human LRIG2 shares 94%, 93% and 92% aa sequence identity with mouse, rat and macaque LRIG2 respectively.

Long Name

Leucine-rich Repeats and Immunoglobulin-like Domains 2

Alternate Names

KIAA0806, LIG2, UFS2

Entrez Gene IDs

9860 (Human); 269473 (Mouse); 310753 (Rat)

Gene Symbol

LRIG2

UniProt

O94898

Additional LRIG2 Products

Product Documents for Human LRIG2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LRIG2 Antibody

For research use only

Related Research Areas

EGF Family

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Protocols

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