Human LRP-1B Antibody Summary
Accession # Q9NZR2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human LRP‑1B by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Human LRP‑1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6379) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LRP-1B (Low density lipoprotein-related protein #1B; also LRP-DIT) is a 600-620 kDa member of the LDLR family of proteins. It is expressed on vascular smooth muscle cells and likely neurons, and binds multiple molecules, including uPA, PAI-1, PDGFR beta uPAR, and Pseudomonas exotoxin. It is considered a tumor suppressor, and appears to block the generation of plasmin. Mature human LRP-1B is a 4579 amino acid (aa) type I transmembrane (TM) glycoprotein. It contains a 4424 aa extracellular domain (ECD) (aa 21-4444) plus a 132 aa cytoplasmic region (aa 4468-4599). In the ECD, there are 32 LDLR class A repeats, 14 EGF-like repeats, and 36 LDLR class B repeats that show an interspersing pattern. In the cytoplasmic region, one NLS (aa 4468-4474) and two endocytosis signal motifs (aa 4492-4562) are found. LRP-B1 is cleaved in the Golgi by furin after Arg3957 to create a noncovalent heterodimer consisting of a 515 kDa ECD, and an 85-95 kDa TM segment. Either uncleaved LRP-1B, or the dissociated heterodimer can undergo additional processing, leading to cleavage of the 90 kDa TM segment after Lys4435. This creates a 21 kDa TM C-terminal fragment that can undergo further gamma -secretase processing to generate a cytoplasmic, 18 kDa peptide that translocates to the nucleus. Over aa 21-244, human LRP-1B shares 81% aa identity with mouse LRP-1B.
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