Human LRP-1B Antibody

Catalog # Availability Size / Price Qty
Detection of Human LRP‑1B by Western Blot.
1 Image
Product Details
Supplemental Products
Reviews (1)

Human LRP-1B Antibody Summary

Species Reactivity
Detects human LRP-1B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP‑1, rhLRP‑4, rhLRP‑5, and rhLRP‑6 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human LRP-1B
Accession # Q9NZR2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LRP-1B antibody by Western Blot. View Larger

Detection of Human LRP‑1B by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Human LRP-1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6379) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-1B

LRP-1B (Low density lipoprotein-related protein #1B; also LRP-DIT) is a 600-620 kDa member of the LDLR family of proteins. It is expressed on vascular smooth muscle cells and likely neurons, and binds multiple molecules, including uPA, PAI-1, PDGFR beta  uPAR, and Pseudomonas exotoxin. It is considered a tumor suppressor, and appears to block the generation of plasmin. Mature human LRP-1B is a 4579 amino acid (aa) type I transmembrane (TM) glycoprotein. It contains a 4424 aa extracellular domain (ECD) (aa 21-4444) plus a 132 aa cytoplasmic region (aa 4468-4599). In the ECD, there are 32 LDLR class A repeats, 14 EGF-like repeats, and 36 LDLR class B repeats that show an interspersing pattern. In the cytoplasmic region, one NLS (aa 4468-4474) and two endocytosis signal motifs (aa 4492-4562) are found. LRP-B1 is cleaved in the Golgi by furin after Arg3957 to create a noncovalent heterodimer consisting of a 515 kDa ECD, and an 85-95 kDa TM segment. Either uncleaved LRP-1B, or the dissociated heterodimer can undergo additional processing, leading to cleavage of the 90 kDa TM segment after Lys4435. This creates a 21 kDa TM C-terminal fragment that can undergo further gamma -secretase processing to generate a cytoplasmic, 18 kDa peptide that translocates to the nucleus. Over aa 21-244, human LRP-1B shares 81% aa identity with mouse LRP-1B.

Long Name
LDL Receptor-related Protein 1B
Entrez Gene IDs
53353 (Human); 94217 (Mouse)
Alternate Names
EC; EC; low density lipoprotein receptor related protein-deleted in tumor; low density lipoprotein receptor-related protein 1B; low density lipoprotein-related protein 1B (deleted in tumors); Low-density lipoprotein receptor-related protein-deleted in tumor; LRP1B; LRP-1B; LRP-deleted in tumors; LRPDIT; LRP-DIT; LRP-DITlow-density lipoprotein receptor-related protein 1B

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human LRP-1B Antibody

Average Rating: 3 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human LRP-1B Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human LRP-1B Antibody
By Anonymous on 07/17/2017
Application: WB Sample Tested: CSF,Human CSF Species: Human