Human LRP-1B Antibody

Catalog # Availability Size / Price Qty
AF6379
AF6379-SP
Human LRP-1B Antibody in Western Blot
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Human LRP-1B Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRP-1B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) LRP‑1, rhLRP‑4, rhLRP‑5, and rhLRP‑6 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LRP-1B
Leu21-Glu244
Accession # Q9NZR2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LRP‑1B by Western Blot. View Larger

Detection of Human LRP‑1B by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Human LRP‑1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6379) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-1B

LRP-1B (Low density lipoprotein-related protein #1B; also LRP-DIT) is a 600-620 kDa member of the LDLR family of proteins. It is expressed on vascular smooth muscle cells and likely neurons, and binds multiple molecules, including uPA, PAI-1, PDGFR beta  uPAR, and Pseudomonas exotoxin. It is considered a tumor suppressor, and appears to block the generation of plasmin. Mature human LRP-1B is a 4579 amino acid (aa) type I transmembrane (TM) glycoprotein. It contains a 4424 aa extracellular domain (ECD) (aa 21-4444) plus a 132 aa cytoplasmic region (aa 4468-4599). In the ECD, there are 32 LDLR class A repeats, 14 EGF-like repeats, and 36 LDLR class B repeats that show an interspersing pattern. In the cytoplasmic region, one NLS (aa 4468-4474) and two endocytosis signal motifs (aa 4492-4562) are found. LRP-B1 is cleaved in the Golgi by furin after Arg3957 to create a noncovalent heterodimer consisting of a 515 kDa ECD, and an 85-95 kDa TM segment. Either uncleaved LRP-1B, or the dissociated heterodimer can undergo additional processing, leading to cleavage of the 90 kDa TM segment after Lys4435. This creates a 21 kDa TM C-terminal fragment that can undergo further gamma -secretase processing to generate a cytoplasmic, 18 kDa peptide that translocates to the nucleus. Over aa 21-244, human LRP-1B shares 81% aa identity with mouse LRP-1B.

Long Name
LDL Receptor-related Protein 1B
Entrez Gene IDs
53353 (Human); 94217 (Mouse)
Alternate Names
EC 1.1.1.94; EC 3.4.21.9; low density lipoprotein receptor related protein-deleted in tumor; low density lipoprotein receptor-related protein 1B; low density lipoprotein-related protein 1B (deleted in tumors); Low-density lipoprotein receptor-related protein-deleted in tumor; LRP1B; LRP-1B; LRP-deleted in tumors; LRPDIT; LRP-DIT; LRP-DITlow-density lipoprotein receptor-related protein 1B

Product Datasheets

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Reviews for Human LRP-1B Antibody

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Human LRP-1B Antibody
By Anonymous on 07/17/2017
Application: WB Sample Tested: Human CSF,CSF Species: Human