|Detection of LRP‑4 in U‑87 MG Human Cell Line by Flow Cytometry. U‑87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human LRP‑4 PE‑conjugated Monoclonal Antibody (Catalog # FAB59481P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.|
LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905). In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 88% aa sequence identity with mouse LRP‑4.