Human LRRC4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LRRC4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human LRRC3 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human LRRC4
    Ala39-Lys527
    Accession # Q9HBW1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human LRRC4
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
LRRC4 in Human Brain. LRRC4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Sheep Anti-Human LRRC4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4995) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRC4

LRRC4 (Leu-rich repeat/LRR-containing protein 4; also brain tumor-associated protein) is a 55 kDa (predicted) member of the Leu-rich protein family. It is expressed on neurons and astrocytes, and antagonizes the signaling pathways for cytokines and growth factors. It is reduced in expression in tumors. Human LRRC4 is a type I transmembrane protein that is 653 amino acids (aa) in length. It contains a 489 aa extracellular region (aa 39-527), and a 105 aa cytoplasmic domain. The extracellular region shows nine LRRs (aa 74-288), a C2 type Ig-like domain (aa 354-440), and a Thr rich segment (aa 455-526). Over aa 39-527, human LRRC4 is 98% aa identical to both mouse and canine LRRC4, respectively.

  • Long Name:
    Leucine-rich Repeat Containing 4
  • Entrez Gene IDs:
    64101 (Human); 192198 (Mouse); 641521 (Rat)
  • Alternate Names:
    BAG; Brain Tumor Associated Protein LRRC4; Brain Tumor-Associated Protein BAG; Leucine Rich Repeat Containing 4; Leucine-Rich Repeat-Containing 4; Leucine-Rich Repeat-Containing Protein 4; LRRC4; NAG14; Nasopharyngeal Carcinoma-Associated Gene 14 Protein; Netrin-G2 Ligand; NGL-2
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