Human Lymphotoxin-alpha /TNF-beta Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF211
Product Details
Citations (3)
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Human Lymphotoxin-alpha /TNF-beta Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lymphotoxin‑ alpha /TNF‑ beta in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) Lymphotactin  alpha 1/ beta 2 is observed and less than 0.1% cross-reactivity with rhTNF-alpha, rmTNF-alpha, rhTNF RI, and rhTNF RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Lymphotoxin‑ alpha /TNF‑ beta
Leu35-Leu205
Accession # P01374
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta (Catalog # 211-TB)

Human Lymphotoxin-alpha /TNF-beta Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Lymphotoxin‑ alpha /TNF‑ beta Antibody (Catalog # MAB621R)

Capture Reagent: Human Lymphotoxin‑ alpha /TNF‑ beta Antibody (Catalog # MAB621)

Standard: Recombinant Human Lymphotoxin-alpha/TNF-beta Protein (Catalog # 211-TBB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin-alpha/TNF-beta

Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex.

TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.

Long Name
Tumor Necrosis Factor beta
Entrez Gene IDs
4049 (Human); 16992 (Mouse)
Alternate Names
LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1

Product Datasheets

Citations for Human Lymphotoxin-alpha /TNF-beta Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lymphotoxin-alphabeta heterotrimers are cleaved by metalloproteinases and contribute to synovitis in rheumatoid arthritis.
    Authors: Young J, Yu X, Wolslegel K
    Cytokine, 2010;51(1):78-86.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  2. A comprehensive ex vivo functional analysis of human NKT cells reveals production of MIP1-alpha and MIP1-beta, a lack of IL-17, and a Th1-bias in males.
    Authors: Snyder-Cappione JE, Tincati C, Eccles-James IG, Cappione AJ, Ndhlovu LC, Koth LL, Nixon DF
    PLoS ONE, 2010;5(11):e15412.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISpot Development
  3. Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
    Authors: Chen DS, Soen Y, Stuge TB
    PLoS Med., 2005;2(10):e265.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay

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