Human Lysine (K)-specific Demethylase 4C/KDM4C Antibody

R&D Systems | Catalog # AF6430

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Lysine (K)-specific Demethylase 4C/KDM4C Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KDM4C
Lys381-Lys588
Accession # Q9H3R0

Specificity

Detects human KDM4C in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Lysine (K)-specific Demethylase 4C/KDM4C Antibody

Detection of Human Lysine (K)-specific Demethylase 4C/KDM4C antibody by Western Blot.

Detection of Human Lysine (K)‑specific Demethylase 4C/KDM4C by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human Lysine (K)-specific Demethylase 4C/KDM4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysine (K)-specific Demethylase 4C/KDM4C at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
KDM4C antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

KDM4C in HeLa Human Cell Line.

KDM4C was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human KDM4C Affinity-purified Polyclonal Antibody (Catalog # AF6430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Lysine (K)-specific Demethylase 4C/KDM4C Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lysine (K)-specific Demethylase 4C/KDM4C

KDM4C (Lysine [K]-specific demethylase 4C; also GASC1 and JMJD2C) is a 120-140 kDa member of the JHDM3 histone demethylase family of enzymes. It is ubiquitously expressed, and specifically demethylates trimethylated lysines on histone H3 (on Lys9 and Lys36), as well as multiple non-histone methylated proteins such as WIZ, CDYL1 and G9a. Transcriptional activation occurs when it interacts with the androgen and progesterone receptors. Human KDM4C is 1056 amino acids (aa) in length. It contains one jumonji N domain (aa 16-58), a catalytic jumonji C domain (aa 144-310), two PHD zinc finger regions (aa 689-865), and two TUDOR methylated-histone binding domains (aa 877-991). Three potential splice variants are reported. One demonstrates an alternative start site 23 aa upstream of the standard site, a second shows a 49 aa substitution for aa 999-1056, and a third contains a five aa substitution for aa 809-1056. Over aa 381-588, human KDM4C shares 51% aa identity with mouse KDM4C.

Alternate Names

GASC1, JHDM3C, JMJD2C, KDM4C, Lysine (K)specific Demethylase 4C

Entrez Gene IDs

23081 (Human); 76804 (Mouse); 298144 (Rat)

Gene Symbol

KDM4C

UniProt

Q9H3R0 (Human)

Additional Lysine (K)-specific Demethylase 4C/KDM4C Products

Product Documents for Human Lysine (K)-specific Demethylase 4C/KDM4C Antibody

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Product Specific Notices for Human Lysine (K)-specific Demethylase 4C/KDM4C Antibody

For research use only

Related Research Areas

Epigenetics

Citations for Human Lysine (K)-specific Demethylase 4C/KDM4C Antibody

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Protocols

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