Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MAGP-2/MFAP5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MAGP-2/MFAP5
Ile22-Leu173
Accession # Q13361

Specificity

Detects human MAGP-2/MFAP5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human MAGP-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human MAGP‑2/MFAP5 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human MAGP-2/MFAP5

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAGP-2/MFAP5

MAGP-2 (Microfibril-associated glycoprotein 2; also MFAP-5) is a 25 kDa member of the MFAP family of proteins. It is secreted by smooth muscle cells and has multiple functions. Through its binding to EGF repeats, MAGP-2 mediates the release of Jagged1 and Notch extracellular domains, and also binds to matrix microfibrillar proteins, inducing elastin fiber formation. Mature human MAGP-2 is 152 amino acids (aa) in length. It contains an RDG motif (aa 30‑32) and a central Cys-rich domain (aa 84‑140) that participates in covalent binding to fibrillin microfibrils. One potential splice variant exists that shows a 10 aa deletion between aa 73‑82. Mature human MAGP-2 is 86% and 87% aa identical to mouse and canine MAGP-2, respectively.

Long Name

Microfibril-associated Glycoprotein 2

Alternate Names

MAGP2, MFAP5, MP25

Entrez Gene IDs

8076 (Human); 50530 (Mouse); 362429 (Rat)

Gene Symbol

MFAP5

UniProt

Q13361 (Human)

Additional MAGP-2/MFAP5 Products

Product Documents for Human MAGP‑2/MFAP5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MAGP‑2/MFAP5 Antibody

For research use only

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail