Human MARCO Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MARCO in direct ELISAs and Western blots. In direct ELISA, approximately 10% cross-reactivity with recombinant mouse MARCO is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human MARCO
    Met79-Val520
    Accession # Q9UEW3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MARCO by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, human lung tissue, and human liver tissue untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human MARCO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MARCO at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MARCO

MARCO (Macrophage receptor with collagenous structure; also scavenger receptor class A member 2/SR-AII) is a 65-70 kDa pattern recognition receptor/PRR that belongs to class A of the SR family, SRCR superfamily of molecules. It is expressed by macrophages, monocytes and Kupffer cells, and apparently binds to a number of microbes, including Gram+ bacteria (Staph and Strep) and Gram- bacteria (Neisseria and coliforms), plus crystalline silica. Human MARCO is a 520 amino acid (aa) type II tranamembrane glycoprotein. It contains a 43 aa cytoplasmic region and a 456 aa extracellular domain/ECD (aa 65-520). The ECD possesses one collagen-like region (aa 147-419) and an SRCR domain (aa 424-519). Human MARCO is believed to form trimers and/or multimers, and these are likely noncovalent associations. Over aa 79-520, human and mouse MARCO share 70% aa sequence identity.

  • Long Name:
    Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
  • Entrez Gene IDs:
    8685 (Human); 17167 (Mouse)
  • Alternate Names:
    macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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