Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MARCO Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MARCO
Met79-Val520
Accession # Q9UEW3

Specificity

Detects human MARCO in direct ELISAs and Western blots. In direct ELISA, approximately 10% cross-reactivity with recombinant mouse MARCO is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human MARCO Antibody

Detection of Human MARCO antibody by Western Blot.

Detection of Human MARCO by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, human lung tissue, and human liver tissue untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human MARCO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MARCO at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human MARCO Antibody

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line, treated with PMA, human lung tissue, and human liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MARCO

MARCO (Macrophage receptor with collagenous structure; also scavenger receptor class A member 2/SR-AII) is a 65-70 kDa pattern recognition receptor/PRR that belongs to class A of the SR family, SRCR superfamily of molecules. It is expressed by macrophages, monocytes and Kupffer cells, and apparently binds to a number of microbes, including Gram+ bacteria (Staph and Strep) and Gram- bacteria (Neisseria and coliforms), plus crystalline silica. Human MARCO is a 520 amino acid (aa) type II tranamembrane glycoprotein. It contains a 43 aa cytoplasmic region and a 456 aa extracellular domain/ECD (aa 65-520). The ECD possesses one collagen-like region (aa 147-419) and an SRCR domain (aa 424-519). Human MARCO is believed to form trimers and/or multimers, and these are likely noncovalent associations. Over aa 79-520, human and mouse MARCO share 70% aa sequence identity.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Alternate Names

SCARA2

Entrez Gene IDs

8685 (Human); 17167 (Mouse)

Gene Symbol

MARCO

UniProt

Q9UEW3

Additional MARCO Products

Product Documents for Human MARCO Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MARCO Antibody

For research use only

Citations for Human MARCO Antibody

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