Detection of Human MARCO by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, human lung tissue, and human liver tissue untreated (-) or treated (+) with 80 nM PMA for 72 hours. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human MARCO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MARCO at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
MARCO (Macrophage receptor with collagenous structure; also scavenger receptor class A member 2/SR-AII) is a 65-70 kDa pattern recognition receptor/PRR that belongs to class A of the SR family, SRCR superfamily of molecules. It is expressed by macrophages, monocytes and Kupffer cells, and apparently binds to a number of microbes, including Gram+ bacteria (Staph and Strep) and Gram- bacteria (Neisseria and coliforms), plus crystalline silica. Human MARCO is a 520 amino acid (aa) type II tranamembrane glycoprotein. It contains a 43 aa cytoplasmic region and a 456 aa extracellular domain/ECD (aa 65-520). The ECD possesses one collagen-like region (aa 147-419) and an SRCR domain (aa 424-519). Human MARCO is believed to form trimers and/or multimers, and these are likely noncovalent associations. Over aa 79-520, human and mouse MARCO share 70% aa sequence identity.
Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2
Entrez Gene IDs:
8685 (Human); 17167 (Mouse)
macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2
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