Human Matriptase/ST14 Catalytic Domain Antibody
Human Matriptase/ST14 Catalytic Domain Antibody Summary
Val615-Val855
Accession # Q9Y5Y6
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Matriptase/ST14 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Matriptase/ST14 Catalytic Domain Monoclonal Antibody (Catalog # MAB3946, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Matriptase/ST14
Human Matriptase, encoded by the ST14 (suppression of tumorogenicity 14) gene, is also known as tumor associated differentially expressed gene 15 protein/TADG‑15), epithin, and membrane-type serine protease 1/MT-SP1 (1). Predicted to have a significant role in tumor biology, Matriptase may be a novel target for anti-cancer therapy (2). However, expressed in most human epithelia, Matriptase is also important in several physiological processes (1). For example, it activates prostasin to initiate a protease cascade that is essential for epidermal differentiation (3), and it converts a single-chain IGFBP-rp1 into the two-chain form (4). Matriptase is a type II transmembrane serine protease with a complex modular structure (1). The 855 amino acid (aa) sequence of human Matriptase consists of a cytoplasmic tail (aa 1‑55), a transmembrane domain (aa 56‑76), and an extracellular portion (aa 77‑855). The latter contains the following domains: SEA (aa 86‑201), two CUBs (aa 214‑334 and 340‑447), four LDLRAs (aa 452‑486, 487‑523, 524‑560, and 566‑603), and a serine protease (aa 615‑855). The physiological activation of the single-chain zymogen requires the cleavage at the SEA domain within the ER or Golgi, association with HAI-1, which facilitates the transport of the protease to the cell surface, and auto-cleavage at QAR-V(615)VGG (1). The activated Matriptase is inhibited by HAI-1, and the resulting HAI-1 complex can be shed from the cell surface (1).
- List, K. et al. (2006) Mol. Med. 12:1.
- Uhland, K. (2006) Cell. Mol. Life Sci. 63:2968.
- Netzel-Arnett, S. et al. (2006) J. Biol. Chem. 281:32941.
- Ahmed, S. et al. (2006) FEBS J. 273:615.
Product Datasheets
Citations for Human Matriptase/ST14 Catalytic Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Downregulation of matriptase suppresses the PAR?2/PLCgamma2/PKC?mediated invasion and migration abilities of MCF?7 breast cancer cells
Authors: JM Kim, J Park, EM Noh, HK Song, SY Kang, SH Jung, JS Kim, HJ Youn, YR Lee
Oncology reports, 2021;46(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
Nat Commun, 2015;6(0):6776.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Epigenetic Silencing of SPINT2 Promotes Cancer Cell Motility via HGF-MET Pathway Activation in Melanoma.
Authors: Hwang S, Kim H, Min M, Raghunathan R, Panova I, Munshi R, Ryu B
J Invest Dermatol, 2015;135(9):2283-91.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase.
Authors: Clark EB, Jovov B, Rooj AK
J. Biol. Chem., 2010;285(35):27130-43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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