Human Matriptase/ST14 Catalytic Domain Antibody AF3946: R&D Systems

Human Matriptase/ST14 Catalytic Domain Antibody

(12 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Matriptase/ST14 Catalytic Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Matriptase/Epithin is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Matriptase/ST14 Catalytic Domain
    Val615-Val855
    Accession # Q9Y5Y6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Matriptase/ST14 Catalytic Domain
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Matriptase/ST14 Catalytic Domain in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Sheep Anti-Human Matriptase/ST14 Catalytic Domain Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3946, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Matriptase/ST14

Human matriptase, encoded by the ST14 (suppression of tumorogenicity 14) gene, is also known as tumor associated differentially expressed gene 15 protein/TADG‑15), epithin, and membrane-type serine protease 1/MT-SP1 (1). Predicted to have a significant role in tumor biology, matriptase may be a novel target for anti-cancer therapy (2). However, expressed in most human epithelia, matriptase is also important in several physiological processes (1). For example, it activates prostasin to initiate a protease cascade that is essential for epidermal differentiation (3), and it converts a single-chain IGFBP-rp1 into the two-chain form (4).

Matriptase is a type II transmembrane serine protease with a complex modular structure (1). The 855 amino acid (aa) sequence of human matriptase consists of a cytoplasmic tail (aa 1-55), a transmembrane domain (aa 56-76), and an extracellular portion (aa 77-855). The latter contains the following domains: SEA (aa 86-201), two CUBs (aa 214-334 and 340-447), four LDLRAs (aa 452-486, 487-523, 524-560, and 566-603), and a serine protease (aa 615-855). The physiological activation of the single-chain zymogen requires the cleavage at the SEA domain within the ER or Golgi, association with HAI-1, which facilitates the transport of the protease to the cell surface, and auto-cleavage at QAR-V(615)VGG (1). The activated matriptase is inhibited by HAI-1, and the resulting HAI-1 complex can be shed from the cell surface (1). R&D Systems rhST14 corresponds to the catalytic domain, and is inhibited effectively by rhHAI-1 and rhHAI-2A (R&D Systems, Catalog # 1048-PI and 1106-PI).

  • References:
    1. List, K. et al. (2006) Mol. Med. 12:1.
    2. Uhland, K. (2006) Cell. Mol. Life Sci. 63:2968.
    3. Netzel-Arnett, S. et al. (2006) J. Biol. Chem. 281:32941.
    4. Ahmed, S. et al. (2006) FEBS J. 273:615.
  • Entrez Gene IDs:
    6768 (Human); 19143 (Mouse); 114093 (Rat)
  • Alternate Names:
    EC 3.4.21; Epithin; HAI; Matriptase; Membrane-type serine protease 1; MTSP1; MT-SP1EC 3.4.21.109; prostamin; PRSS14; Serine protease 14; Serine protease TADG-15; SNC19; SNC19MTSP1; ST14; suppression of tumorigenicity 14 (colon carcinoma); suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); suppressor of tumorigenicity 14 protein; TADG15; TADG-15; TMPRSS14; tumor associated differentially expressed gene 15 protein; Tumor-associated differentially-expressed gene 15 protein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Inflammatory cytokines down-regulate the barrier protective prostasin-matriptase proteolytic cascade early in experimental colitis
    Authors: MS Buzza, TA Johnson, GD Conway, EW Martin, S Mukhopadhy, T Shea-Donoh, TM Antalis
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Matriptase zymogen supports epithelial development, homeostasis and regeneration
    Authors: S Friis, D Tadeo, SM Le-Gall, HJ Jürgensen, KU Sales, E Camerer, TH Bugge
    BMC Biol., 2017;15(1):46.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Distinct Developmental Functions of Prostasin (CAP1/PRSS8) Zymogen and Activated Prostasin
    Authors: S Friis, DH Madsen, TH Bugge
    J. Biol. Chem, 2016;291(6):2577-82.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Simple Western
  4. Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors.
    Authors: Sales K, Friis S, Abusleme L, Moutsopoulos N, Bugge T
    Oncogene, 2015;34(35):4664-72.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
    Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K
    Nat Commun, 2015;6(0):6776.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Regulation of feto-maternal barrier by matriptase- and PAR-2-mediated signaling is required for placental morphogenesis and mouse embryonic survival.
    Authors: Szabo R, Peters D, Kosa P, Camerer E, Bugge T
    PLoS Genet, 2014;10(7):e1004470.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Matriptase proteolytically activates influenza virus and promotes multicycle replication in the human airway epithelium.
    Authors: Beaulieu A, Gravel E, Cloutier A, Marois I, Colombo E, Desilets A, Verreault C, Leduc R, Marsault E, Richter M
    J Virol, 2013;87(8):4237-51.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.
    Authors: Szabo, Roman, Uzzun Sales, Katiuchi, Kosa, Peter, Shylo, Natalia, Godiksen, Sine, Hansen, Karina K, Friis, Stine, Gutkind, J Silvio, Vogel, Lotte K, Hummler, Edith, Camerer, Eric, Bugge, Thomas H
    PLoS Genet, 2012;8(8):e1002937.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  9. Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiation.
    Authors: Fang JD, Chou HC, Tung HH
    J. Biol. Chem., 2011;286(7):5667-79.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  10. c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
    Authors: Szabo R, Rasmussen AL, Moyer AB
    Oncogene, 2011;30(17):2003-16.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  11. Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome.
    Authors: Sales KU, Masedunskas A, Bey AL
    Nat. Genet., 2010;42(8):676-83.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  12. Loss of matriptase suppression underlies spint1 mutation-associated ichthyosis and postnatal lethality.
    Authors: Szabo R, Kosa P, List K, Bugge TH
    Am. J. Pathol., 2009;174(6):2015-22.
    Species: Mouse
    Sample Type: Recombinant Protein
    Application: WB
Expand to show all 12 Citations
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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