Human MICA Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1300
Human MICA Biotinylated Antibody in Data
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Human MICA Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross reactivity with recombinant human (rh) MICB and rhMIC1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICA
Ala22-Gln308
Accession # NP_001170990
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
Immunohistochemistry
5-15 µg/mL
See below

Human MICA Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human MICA Antibody (Catalog # MAB13002)

Standard: Recombinant Human MICA Fc Chimera Protein, CF (Catalog # 1300-MA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry MICA in Human Colon. View Larger

MICA in Human Colon. MICA was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human MICA Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1300) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry MICA in Human Colon. View Larger

MICA in Human Colon. MICA was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human MICA Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1300) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICA

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

References
  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. NKG2D and its Ligands (2002) http://www.RnDSystems.com.
Long Name
MHC Class I-related Protein A
Entrez Gene IDs
100507436 (Human)
Alternate Names
FLJ60820; MGC111087; MICA; PERB11.1

Product Datasheets

Citations for Human MICA Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
    Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
    Oncotarget, 2017;8(19):31959-31976.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  2. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
    Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
    J Leukoc Biol, 2015;97(3):583-98.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA.
    Authors: Esteso G, Luzon E, Sarmiento E, Gomez-Caro R, Steinle A, Murphy G, Carbone J, Vales-Gomez M, Reyburn H
    J Immunol, 2014;193(3):1344-52.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
    Authors: Nuckel H, Switala M, Sellmann L, Horn PA, Durig J, Duhrsen U, Kuppers R, Grosse-Wilde H, Rebmann V
    Leukemia, 2010;24(6):1152-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  5. Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS.
    Authors: Guerra S, Gonzalez JM, Climent N, Reyburn H, Lopez-Fernandez LA, Najera JL, Gomez CE, Garcia F, Gatell JM, Gallart T, Esteban M
    J. Virol., 2010;84(16):8141-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.
    Authors: Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A
    Blood, 2007;111(3):1428-36.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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Immunohistochemistry Reagents

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Staining Reagents

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Reviews for Human MICA Biotinylated Antibody

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Human MICA Biotinylated Antibody
By Anonymous on 04/21/2017
Application: IHC Sample Tested: Breast cancer tissue Species: Human

Antigen retrieval at pH 6.0 (20min).
MICA BAF1300:
Dilution 1:100
Incubation-1h
Detection with ImmPRESS HRP Anti-Goat Ig (Peroxidase) Polymer