Human MICA Biotinylated Antibody
Human MICA Biotinylated Antibody Summary
Ala22-Gln308
Accession # NP_001170990
Applications
Human MICA Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
MICA in Human Colon. MICA was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human MICA Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1300) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
View Larger
MICA in Human Colon. MICA was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human MICA Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1300) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MICA
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
- Groh, V. et al. (2001) Nature Immunol. 2:255.
- Stephens, H. (2001) Trends Immunol. 22:378.
- Bauer, S. et al. (1999) Science 285:727.
- Groh, V. et al. (2002) Nature 419:734.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Pende, D. et al. (2002) Cancer Res. 62:6178.
- NKG2D and its Ligands (2002) http://www.RnDSystems.com.
Product Datasheets
Citations for Human MICA Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
Filter your results:
Filter by:
-
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
Oncotarget, 2017;8(19):31959-31976.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
J Leukoc Biol, 2015;97(3):583-98.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA.
Authors: Esteso G, Luzon E, Sarmiento E, Gomez-Caro R, Steinle A, Murphy G, Carbone J, Vales-Gomez M, Reyburn H
J Immunol, 2014;193(3):1344-52.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
Authors: Nuckel H, Switala M, Sellmann L, Horn PA, Durig J, Duhrsen U, Kuppers R, Grosse-Wilde H, Rebmann V
Leukemia, 2010;24(6):1152-9.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS.
Authors: Guerra S, Gonzalez JM, Climent N, Reyburn H, Lopez-Fernandez LA, Najera JL, Gomez CE, Garcia F, Gatell JM, Gallart T, Esteban M
J. Virol., 2010;84(16):8141-52.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.
Authors: Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A
Blood, 2007;111(3):1428-36.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Staining Reagents
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Human MICA Biotinylated Antibody
Average Rating: 3 (Based on 1 Review)
Have you used Human MICA Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Antigen retrieval at pH 6.0 (20min).
MICA BAF1300:
Dilution 1:100
Incubation-1h
Detection with ImmPRESS HRP Anti-Goat Ig (Peroxidase) Polymer
