Human MICA Antibody

Catalog #: MAB13002
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13002-SP
MAB13002-500
MAB13002-100

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Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MICA Antibody Summary

Species Reactivity

Human

Specificity

Detects human MICA in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MICB is observed.

Source

Monoclonal Mouse IgG₁ Clone # 159233

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
under non-reducing conditions only

Human MICA Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human MICA Biotinylated Antibody (Catalog # BAF1300)

Standard: Recombinant Human MICA Fc Chimera Protein, CF (Catalog # 1300-MA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICA

MHC class I Chain-related gene A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

Long Name

MHC Class I-related Protein A

Entrez Gene IDs

100507436 (Human)

Alternate Names

FLJ60820; MGC111087; MICA; PERB11.1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Natural Killer T (NKT) Cells

Citation for Human MICA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS.
Authors: Guerra S, Gonzalez JM, Climent N, Reyburn H, Lopez-Fernandez LA, Najera JL, Gomez CE, Garcia F, Gatell JM, Gallart T, Esteban M
J. Virol., 2010;84(16):8141-52.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development