Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 159233
View all MICA Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983

Specificity

Detects human MICA in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MICB is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human MICA Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
under non-reducing conditions only

Human MICA Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human MICA Biotinylated Antibody (Catalog # BAF1300)
  • Standard: Recombinant Human MICA Fc Chimera Protein, CF (Catalog # 1300-MA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MICA

MHC class I Chain-related gene A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

Long Name

MHC Class I-related Protein A

Alternate Names

FLJ60820, MGC111087, PERB11.1

Entrez Gene IDs

100507436 (Human)

Gene Symbol

MICA

UniProt

Q29983

Additional MICA Products

Product Documents for Human MICA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MICA Antibody

For research use only

Related Research Areas

Natural Killer T (NKT) Cells

Citations for Human MICA Antibody

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FAQs

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