MHC class I Chain-related gene A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
Human MICA Antibody (159233)
R&D Systems | Catalog # MAB13002
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Western Blot, Immunocytochemistry, ELISA Capture, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 159233
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Product Specifications
Immunogen
BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983
Accession # Q29983
Specificity
Detects human MICA in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MICB is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human MICA Antibody (159233)
Human MICA ELISA Standard Curve
Recombinant Human MICA Fc Chimera (Catalog # 1300-MA) was serially diluted and captured by Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB13002) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MICA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1300) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human MICA Antibody (159233)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
under non-reducing conditions only
Sample: Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
under non-reducing conditions only
Human MICA Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MICA
Long Name
MHC Class I-related Protein A
Alternate Names
FLJ60820, MGC111087, PERB11.1
Entrez Gene IDs
100507436 (Human)
Gene Symbol
MICA
UniProt
Additional MICA Products
Product Documents for Human MICA Antibody (159233)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MICA Antibody (159233)
For research use only
Related Research Areas
Citations for Human MICA Antibody (159233)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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