Detects human MICA in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human MICB is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human MICA Ala23-Gln308 Accession # NP_001170990
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.4-1.2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of biotinylated Recombinant Human MICA Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299‑NK) coated at 2 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
MICA in Human Breast Cancer Tissue. MICA was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human MICA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1300) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
Groh, V. et al. (2001) Nature Immunol. 2:255.
Stephens, H. (2001) Trends Immunol. 22:378.
Bauer, S. et al. (1999) Science 285:727.
Groh, V. et al. (2002) Nature 419:734.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Pende, D. et al. (2002) Cancer Res. 62:6178.
NKG2D and its Ligands (2002) http://www.RnDSystems.com
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.