Detects recombinant human (rh) MMP-12 in Western blots. Recognizes forms of rhMMP-12 containing the C-terminal hemopexin-like domain only. Does not detect the fully mature 20 kDa proteolytic MMP-12 entity, where the C-terminal domain has been lost.
Monoclonal Mouse IgG1 Clone # 4D2
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human MMP-12
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human MMP‑12 by Western Blot.
Western blot shows lysates of human lung tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human MMP‑12 Hemopexin Domain Monoclonal Antibody (Catalog # MAB917) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑12 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MMP‑12 in Human Ovarian Cancer Tissue.
MMP‑12 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑12 Hemopexin Domain Monoclonal Antibody (Catalog # MAB917) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MMP-12, also known as macrophage elastase, is a zinc and calcium
dependent endopeptidase expressed constitutively in macrophages and can
be induced in monocytes. MMP-12 can degrade a broad spectrum of
substrates, including type IV collagen, fibronectin, laminin,
vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein,
alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3.
The MMP-12 precursor consists of a pro domain, a catalytic domain
containing the zinc-binding site, and a C-terminal hemopexin-like
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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