Detects recombinant human (rh) MMP-12 in Western blots. Recognizes forms of rhMMP-12 containing the C-terminal hemopexin-like domain only. Does not detect the fully mature 20 kDa proteolytic MMP-12 entity, where the C-terminal domain has been lost.
Monoclonal Mouse IgG1 Clone # 4D2
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human MMP-12
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human MMP‑12 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human MMP‑12 Hemopexin Domain Monoclonal Antibody (Catalog # MAB917) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑12 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MMP‑12 in Human Ovarian Cancer Tissue. MMP‑12 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑12 Hemopexin Domain Monoclonal Antibody (Catalog # MAB917) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MMP-12, also known as macrophage elastase, is a zinc and calcium
dependent endopeptidase expressed constitutively in macrophages and can
be induced in monocytes. MMP-12 can degrade a broad spectrum of
substrates, including type IV collagen, fibronectin, laminin,
vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein,
alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3.
The MMP-12 precursor consists of a pro domain, a catalytic domain
containing the zinc-binding site, and a C-terminal hemopexin-like
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.