Human MMP-12 Hemopexin Domain Antibody

MAB917 has been discontinued. View all MMP-12 products.
(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects recombinant human (rh) MMP-12 in Western blots. Recognizes forms of rhMMP-12 containing the C-terminal hemopexin-like domain only. Does not detect the fully mature 20 kDa proteolytic MMP-12 entity, where the C-terminal domain has been lost.
  • Source
    Monoclonal Mouse IgG1 Clone # 4D2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human MMP-12
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MMP‑12 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human MMP‑12 Hemopexin Domain Monoclonal Antibody (Catalog # MAB917) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP‑12 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
MMP‑12 in Human Ovarian Cancer Tissue. MMP‑12 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑12 Hemopexin Domain Monoclonal Antibody (Catalog # MAB917) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-12
MMP-12, also known as macrophage elastase, is a zinc and calcium dependent endopeptidase expressed constitutively in macrophages and can be induced in monocytes. MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. The MMP-12 precursor consists of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain.
  • Long Name:
    Matrix Metalloproteinase 12
  • Entrez Gene IDs:
    4321 (Human); 17381 (Mouse)
  • Alternate Names:
    EC 3.4.24; hME; HMEEC 3.4.24.65; Macrophage elastase; macrophage metalloelastase; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP12; MMP-12
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Cell-type deconvolution with immune pathways identifies gene networks of host defense and immunopathology in leprosy
    JCI Insight, 2016;1(15):e88843.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  2. GPI-anchored TIMP-1 treatment renders renal cell carcinoma sensitive to FAS-meditated killing.
    Authors: Djafarzadeh R, Noessner E, Engelmann H
    Oncogene, 2006;25(10):1496-508.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB

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