Human MMP-15/MT2-MMP PE-conjugated Antibody

IC9161P has been discontinued. View all MMP-15/MT2-MMP products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human MMP‑15/MT2‑MMP in direct ELISAs and Western blots. Does not detect the catalytic domain of E. coli-expressed recombinant human MMP-15 (aa 132‑304).
  • Source
    Monoclonal Mouse IgG1 Clone # 130522
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MMP-15/MT2-MMP
    aa 132-625
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of MMP‑15/MT2‑MMP in A375 Human Cell Line by Flow Cytometry. A375 human melanoma cell line was stained with Mouse Anti-Human MMP‑15/MT2‑MMP PE‑conjugated Monoclonal Antibody (Catalog # IC9161P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MMP-15/MT2-MMP
MMP-15, also known as Membrane-Type 2 (MT2) MMP, is a cell surface endopeptidase that plays a role in tumor invasion and metastasis.
  • Long Name:
    Matrix Metalloproteinase 15/Membrane Type 2 MMP
  • Entrez Gene IDs:
    4324 (Human)
  • Alternate Names:
    EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 15 (membrane-inserted); matrix metalloproteinase 15 (membrane-inserted); Membrane-type matrix metalloproteinase 2; Membrane-type-2 matrix metalloproteinase; MMP15; MMP-15; MT2-MMP; MT2-MMPMT2MMP; MTMMP2MT-MMP 2; SMCP-2matrix metalloproteinase-15
Related Research Areas

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