Detection of MMP‑15/MT2‑MMP in A375 Human Cell Line by Flow Cytometry. A375 human melanoma cell line was stained with Mouse Anti-Human MMP‑15/MT2‑MMP PE‑conjugated Monoclonal Antibody (Catalog # IC9161P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
MMP-15, also known as Membrane-Type 2 (MT2) MMP, is a cell surface endopeptidase that plays a role in tumor invasion and metastasis.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.