Human MMP-9 DuoSet ELISA

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Ancillary Products Available
Human MMP-9 ELISA Standard Curve
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Product Details
Citations (32)
Supplemental Products
Reviews (5)

Human MMP-9 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MMP-9. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human MMP-9 ELISA Standard Curve

Product Datasheets

Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat)
Alternate Names:
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

Citations for Human MMP-9 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Evaluation of the post-treatment anti-inflammatory capacity of osteoarthritic chondrocytes: An in�vitro study using baicalein
    Authors: CC Wu, YR Chen, DH Lu, LH Hsu, KC Yang, S Sumi
    Regen Ther, 2020;14(0):177-183.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Plasma levels of matrix metalloproteinase-9 are elevated in individuals with hypertensive crisis
    Authors: FM Valente, DO de Andrade, LN Cosenso-Ma, CB Cesarino, SM Guimarães, VB Guimarães, R Lacchini, JE Tanus-Sant, JC Yugar-Tole, JF Vilela-Mar
    BMC Cardiovasc Disord, 2020;20(1):132.
    Species: Human
    Sample Types: Plasma
  3. Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants
    Authors: YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park
    Invest. Ophthalmol. Vis. Sci., 2019;60(12):3813-3820.
    Species: Human
    Sample Types: Plasma
  4. Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response
    Authors: S Coufal, N Galanova, L Bajer, Z Gajdarova, D Schierova, Z Jiraskova, K Kostovciko, Z Jackova, Z Stehlikova, P Drastich, H Tlaskalova, M Kverka
    Cells, 2019;8(7):.
    Species: Human
    Sample Types: Serum
  5. Neutrophil Dysfunction in the Airways of Children with Acute Respiratory Failure Due to Lower Respiratory Tract Viral and Bacterial Coinfections
    Authors: JR Grunwell, VD Giacalone, S Stephenson, C Margaroli, BS Dobosh, MR Brown, AM Fitzpatric, R Tirouvanzi
    Sci Rep, 2019;9(1):2874.
    Species: Human
    Sample Types: Plasma
  6. Proteolytic biomarkers are related to prognosis in COPD- report from a population-based cohort
    Authors: R Linder, E Rönmark, J Pourazar, AF Behndig, A Blomberg, A Lindberg
    Respir. Res., 2018;19(1):64.
    Species: Human
    Sample Types: Serum
  7. Clinical predictors of liver fibrosis in patients with chronic hepatitis B virus infection from children to adults
    Authors: JF Wu, SH Song, CS Lee, HL Chen, YH Ni, HY Hsu, TC Wu, MH Chang
    J. Infect. Dis., 2018;0(0):.
    Species: Human
    Sample Types: Serum
  8. Streptococcus agalactiae Induces Placental Macrophages To Release Extracellular Traps Loaded with Tissue Remodeling Enzymes via an Oxidative Burst-Dependent Mechanism
    Authors: RS Doster, JA Sutton, LM Rogers, DM Aronoff, JA Gaddy
    MBio, 2018;9(6):.
    Species: Human
    Sample Types:
  9. Plasma inflammatory and immune proteins as predictors of intra-amniotic infection and spontaneous preterm delivery in women with preterm labor: a retrospective study
    Authors: H Park, KH Park, YM Kim, SY Kook, SJ Jeon, HN Yoo
    BMC Pregnancy Childbirth, 2018;18(1):146.
    Species: Human
    Sample Types: Plasma
  10. MicroRNA-320a protects against osteoarthritis cartilage degeneration by regulating the expressions of BMI-1 and RUNX2 in chondrocytes
    Authors: H Peng, D Liang, B Li, C Liang, W Huang, H Lin
    Pharmazie, 2017;72(4):223-226.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard
    Authors: N Askari, T Ghazanfari, MM Naghizadeh, A Moin, A Khamesipou, S Pourfarzam, ZM Hassan
    Iran J Pathol, 2017;12(3):257-264.
    Species: Human
    Sample Types: Serum
  12. Periodontitis and type 2 diabetes among women with previous gestational diabetes: epidemiological and immunological aspects in a follow-up of three years
    Authors: RP Esteves Li, LO Cota, TA Silva, SC Cortelli, JR Cortelli, FO Costa
    J Appl Oral Sci, 2017;25(2):130-139.
    Species: Human
    Sample Types: Saliva
  13. Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
    Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
    Oncol Lett, 2017;13(5):3269-3276.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Clinical significance of histologic chorioamnionitis with a negative amniotic fluid culture in patients with preterm labor and premature membrane rupture
    Authors: JW Park, KH Park, EY Jung
    PLoS ONE, 2017;12(3):e0173312.
    Species: Human
    Sample Types: Amniotic Fluid
  15. Serum Biomarkers of Myocardial Remodeling and Coronary Dysfunction in Early Stages of Hypertrophic Cardiomyopathy in the Young
    Authors: E Fernlund, T Gyllenhamm, R Jablonowsk, M Carlsson, A Larsson, J Ärnlöv, P Liuba
    Pediatr Cardiol, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  16. Development of Combining of Human Bronchial Mucosa Models with XposeALI? for Exposure of Air Pollution Nanoparticles
    Authors: J Ji, A Hedelin, M Malml”f, V Kessler, G Seisenbaev, P Gerde, L Palmberg
    PLoS ONE, 2017;12(1):e0170428.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Serological signatures of clinical cure following successful treatment with sodium stibogluconate in Ethiopian visceral leishmaniasis
    Cytokine, 2016;91(0):6-9.
    Species: Human
    Sample Types: Serum
  18. Association of Matrix Metalloproteinases -7, -8 and -9 and TIMP -1 with Disease Severity in Acute Pancreatitis. A Cohort Study
    PLoS ONE, 2016;11(8):e0161480.
    Species: Human
    Sample Types: Plasma
  19. Therapy with plasma purified alpha1-antitrypsin (Prolastin(R)) induces time-dependent changes in plasma levels of MMP-9 and MPO.
    Authors: Koepke J, Dresel M, Schmid S, Greulich T, Beutel B, Schmeck B, Vogelmeier C, Janciauskiene S, Koczulla A
    PLoS ONE, 2015;10(0):.
    Species: Human
    Sample Types: Serum
  20. Serum metalloproteinase-9 is related to COPD severity and symptoms - cross-sectional data from a population based cohort-study.
    Authors: Linder R, Ronmark E, Pourazar J, Behndig A, Blomberg A, Lindberg A
    Respir Res, 2015;16(0):28.
    Species: Human
    Sample Types: Serum
  21. A novel nonhuman primate model of cigarette smoke-induced airway disease.
    Authors: Polverino F, Doyle-Eisele M, McDonald J, Wilder J, Royer C, Laucho-Contreras M, Kelly E, Divo M, Pinto-Plata V, Mauderly J, Celli B, Tesfaigzi Y, Owen C
    Am J Pathol, 2015;185(3):741-55.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: BALF
  22. In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1beta, TNFalpha, IL-6, IL-8, IL-10, MIP-1alpha, and MMP-9 in pre-labor human term placenta.
    Authors: Garcia-Ruiz G, Flores-Espinosa P, Preciado-Martinez E, Bermejo-Martinez L, Espejel-Nunez A, Estrada-Gutierrez G, Maida-Claros R, Flores-Pliego A, Zaga-Clavellina V
    Reprod Biol Endocrinol, 2015;13(0):115.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Recognition of bacterial signal peptides by mammalian formyl peptide receptors: a new mechanism for sensing pathogens.
    Authors: Bufe B, Schumann T, Kappl R, Bogeski I, Kummerow C, Podgorska M, Smola S, Hoth M, Zufall F
    J Biol Chem, 2015;290(12):7369-87.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Identification of neutrophil activation markers as novel surrogate markers of CF lung disease.
    Authors: Rath T, Zwaschka L, Hage L, Kugler M, Menendez K, Naehrlich L, Schulz R, Roderfeld M, Roeb E
    PLoS ONE, 2014;9(12):e115847.
    Species: Human
    Sample Types: Serum
  25. Compartment differences of inflammatory activity in chronic obstructive pulmonary disease.
    Authors: Ji J, von Scheele I, Bergstrom J, Billing B, Dahlen B, Lantz A, Larsson K, Palmberg L
    Respir Res, 2014;15(0):104.
    Species: Human
    Sample Types: Serum
  26. Controlled and uncontrolled asthma display distinct alveolar tissue matrix compositions.
    Authors: Weitoft M, Andersson C, Andersson-Sjoland A, Tufvesson E, Bjermer L, Erjefalt J, Westergren-Thorsson G
    Respir Res, 2014;15(0):67.
    Species: Human
    Sample Types: BALF
  27. Clinical utility of diagnostic guidelines and putative biomarkers in lymphangioleiomyomatosis.
    Respir. Res., 2012;13(0):34.
    Species: Human
    Sample Types: Serum
  28. Non-acylated Mycobacterium bovis glycoprotein MPB83 binds to TLR1/2 and stimulates production of matrix metalloproteinase 9.
    Authors: Chambers MA, Whelan AO, Spallek R
    Biochem. Biophys. Res. Commun., 2010;400(3):403-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009;125(2):367-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Cerebrospinal fluid B cells correlate with early brain inflammation in multiple sclerosis.
    Authors: Kuenz B, Lutterotti A, Ehling R, Gneiss C, Haemmerle M, Rainer C, Deisenhammer F, Schocke M, Berger T, Reindl M
    PLoS ONE, 2008;3(7):e2559.
    Species: Human
    Sample Types: Serum
  31. Association of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-4 in cerebrospinal fluid with blood-brain barrier dysfunction in patients with eosinophilic meningitis caused by Angiostrongylus cantonensis.
    Authors: Tsai HC, Chung LY, Chen ER, Sy CL
    Am. J. Trop. Med. Hyg., 2008;78(1):20-7.
    Species: Human
    Sample Types: CSF
  32. Matrix metalloproteinases and their tissue inhibitors (TIMPs) in Plasmodium falciparum malaria: serum levels of TIMP-1 are associated with disease severity.
    Authors: Dietmann A, Helbok R, Lackner P, Issifou S, Lell B, Matsiegui PB, Reindl M, Schmutzhard E, Kremsner PG
    J. Infect. Dis., 2008;197(11):1614-20.
    Species: Human
    Sample Types: Serum


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Reviews for Human MMP-9 DuoSet ELISA

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Human MMP-9 DuoSet ELISA
By Anonymous on 09/02/2019
Application: Sample Tested: Tissue Culture Media

Human MMP-9 DuoSet ELISA
By Anonymous on 10/11/2018
Application: Sample Tested: Serum and Plasma

Human MMP-9 DuoSet ELISA
By Anonymous on 07/11/2017
Application: Sample Tested: EDTA Plasma

We ran human EDTA plasma at a 1:400 dilution for this assay. The kit performed ideally with high precision and satisfaction.

Human MMP-9 DuoSet ELISA
By Anonymous on 05/07/2017
Application: Sample Tested: Serum

Human MMP-9 DuoSet ELISA
By Anonymous on 05/02/2017
Application: Sample Tested: Adult lung