MMP-9 (Gelatinase B) is a metalloproteinase that degrades multiple extracellular matrix components including Gelatin, Collagens III, IV, V, and XI, Elastin, Nidogen-1, and Vitronectin. It also cleaves a variety of chemokines and growth factors (e.g. IL-1 beta, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, Latent TGF-beta, membrane bound TNF-alpha, VEGF, and FGF basic), Amyloid beta peptide, Substance P, and Myelin Basic Protein. MMP-9 can trigger signaling through various transmembrane proteins or inhibit signaling by inducing their shedding from the cell surface (e.g. CD44, E-Cadherin, Integrins, ICAM-1, and CD25/IL-2 R alpha). The activity of MMP-9 is regulated by its association with the TIMP-1 protease inhibitor. Circulating levels of MMP-9 are elevated in many inflammatory disorders including intraluminal thrombus formation, atherosclerosis, Crohn’s disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy.
Human MMP-9 DuoSet ELISA
R&D Systems | Catalog # DY911
Key Product Details
Assay Type
Assay Range
Sample Type
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human MMP-9 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human MMP-9 DuoSet ELISA
Product Specifications
Assay Format
Sample Volume Required
Detection Method
Conjugate
Specificity
Label
Scientific Data Images for Human MMP-9 DuoSet ELISA
Human MMP-9 ELISA Standard Curve
Kit Contents for Human MMP-9 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS:
(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
Stability & Storage
Background: MMP-9
Long Name
Alternate Names
Gene Symbol
Additional MMP-9 Products
Product Documents for Human MMP-9 DuoSet ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MMP-9 DuoSet ELISA
For research use only
Citations for Human MMP-9 DuoSet ELISA
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Customer Images
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Sample Tested: Corneal stromal stem cellVerified Customer | Posted 03/01/2022
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Sample Tested: Cell culture supernatantVerified Customer | Posted 12/09/2021Dermal fibroblast cell culture supernatant following stimulation with 2ng/mL TGFb1
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Sample Tested: Cell Culture MediaVerified Customer | Posted 08/19/2021
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Sample Tested: Cell Culture MediaVerified Customer | Posted 08/06/2021
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Sample Tested: Tissue Culture MediaVerified Customer | Posted 09/02/2019
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Sample Tested: Serum and PlasmaVerified Customer | Posted 10/11/2018
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Sample Tested: EDTA PlasmaVerified Customer | Posted 07/11/2017We ran human EDTA plasma at a 1:400 dilution for this assay. The kit performed ideally with high precision and satisfaction.
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Sample Tested: SerumVerified Customer | Posted 05/07/2017
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Sample Tested: Adult lungVerified Customer | Posted 05/02/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
