Human/Mouse 14-3-3 zeta Antibody

Catalog # Availability Size / Price Qty
AF2669
AF2669-SP
Detection of Human/Mouse 14‑3‑3 zeta  by Western Blot.
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Product Details
Citations (2)
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Human/Mouse 14-3-3 zeta Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human, mouse, and rat 14-3-3 zeta in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human 14‑3‑3 beta, epsilon, eta, gamma, or sigma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human 14-3-3 zeta
Met1-Asn245
Accession # P63104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse 14‑3‑3 zeta  by Western Blot. View Larger

Detection of Human/Mouse 14‑3‑3 zeta  by Western Blot.
Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheo­chromocytoma cell line. PVDF membrane was probed with 0.25 µg/mL Goat Anti-Human/
Mouse 14‑3‑3 zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2669) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human 14-3-3 beta, epsilon, eta, gamma, sigma, and zeta (2 ng/lane) were included. A specific band for 14-3-3 zeta was detected at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 14-3-3 zeta

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in high abundance in all eukaryotic cells. 14-3-3 proteins were the first polypeptides shown to have pSer/Thr binding properties, generally recognizing the consensus sequences RSXpSXP and RXY/FXpSXP (where X is any amino acid). 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. For example, the binding of 14-3-3 to phosphorylated BAD blocks its proapoptotic association with Bcl-XL. There are at least seven distinct 14-3-3 genes in vertebrates, alpha/beta, epsilon, eta, gamma, theta, sigma and zeta ( alpha / beta, epsilon, eta, gamma, tau / theta, sigma, and zeta /δ). 14-3-3 zeta, also known as Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, zeta isoform (YWHAZ) is a 245 amino acid, 27 kDa protein.

Entrez Gene IDs
7534 (Human)
Alternate Names
1433 zeta; 14-3-3 zeta; KCIP-1; KCIP-1MGC126532,14-3-3-zeta; MGC111427; MGC138156; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, deltapolypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zetapolypeptide; YWHAZ; zeta polypeptide

Product Datasheets

Citations for Human/Mouse 14-3-3 zeta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. 14-3-3epsilon and zeta regulate neurogenesis and differentiation of neuronal progenitor cells in the developing brain.
    Authors: Toyo-oka K, Wachi T, Hunt R, Baraban S, Taya S, Ramshaw H, Kaibuchi K, Schwarz Q, Lopez A, Wynshaw-Boris A
    J Neurosci, 2014;34(36):12168-81.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  2. Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery.
    Authors: Yu KH, Barry CG, Austin D
    J. Proteome Res., 2009;8(3):1565-76.
    Species: Human
    Sample Types: Serum
    Applications: WB

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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