Human/Mouse Acetyl Histone H4 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects immunogen in direct ELISAs and human Acetyl Histone H4 in Western blots. Reactivity with non-acetylated peptide was removed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Tetrahymena pyriformis acetylated Histone H4 synthetic peptide
    AGGKGGKGMGKVGAKRHSK
    Accession # P02310
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Acetyl Histone H4 by Western Blot. Western blot shows lysates of Tera‑2 human embryonic lung carcinoma cell line, Nalm‑6 human Pre-B acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti‑Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5215) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Acetyl Histone H4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunocytochemistry
Histone H4 in NTera‑2 Human Cell Line. Histone H4 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone H4

Histone 4 (H4/HIST1H4I; also H4M and HIST4H4) is a 12-15 kDa member of the histone H4 family of molecules. It is ubiquitously expressed, with H4 initially synthesized and deposited in replicating chromatin during S phase. H4 undergoes multiple posttranslational modifications. At the G2/M transition, H4 is monomethylated on Lys20 and possibly contributes to transcription initiation/elongation. As the cell moves into the M/G1 phase, Lys20 becomes either dimethylated or trimethylated, contributing to DNA repair and telomere maintenance, respectively. H4 is also known to be phosphorylated on Ser2 and 48, plus Tyr52 and 89, and acetylated on at least nine sites. Human histone 4 is 103 amino acids (aa) in length. Full length histone 4 is identical in aa sequence to mouse histone 4, and 78% aa identical to Tetrahymena histone 4 over aa 2-20 used for immunization.

  • Entrez Gene IDs:
    121504 (Human); 319159 (Mouse)
  • Alternate Names:
    H4; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4F2; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; histone 4, H4; Histone Cluster 1 H4; Histone Cluster 1 H4i; Histone Cluster 4 H4; histone cluster 4, H4; Histone H4; MGC24116
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