Detection of Human/Mouse AMPK beta 2 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse AMPK beta 2 Monoclonal Antibody (Catalog # MAB3808) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human AMPK beta 2 (0.5 ng) was included. A specific band for AMPK beta 2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AMPK beta 2
AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2subunit in liver, while beta 2 is more abundant in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.
AMP-activated Protein Kinase beta 2
Entrez Gene IDs:
5565 (Human); 108097 (Mouse); 64562 (Rat)
5'-AMP-activated protein kinase, beta-2 subunit; AMPK beta 2; AMPK beta 2,5'-AMP-activated protein kinase subunit beta-2; AMPK beta-2 chain; AMPK subunit beta-2; MGC61468; PRKAB2; protein kinase, AMP-activated, beta 2 non-catalytic subunit
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