AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2subunit in liver, while beta 2 is more abundant in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.
AMPK beta 2 Antibody (376938)
R&D Systems | Catalog # MAB3808
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 376938
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Product Specifications
Immunogen
E. coli-derived recombinant human AMPK beta 2
Met1-Ile272
Accession # O43741
Met1-Ile272
Accession # O43741
Specificity
Detects human and mouse AMPK beta 2 in Western blots. No cross-reactivity with recombinant human AMPK beta 1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for AMPK beta 2 Antibody (376938)
Detection of Human/Mouse AMPK beta 2 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL Rat Anti-Human/Mouse AMPK beta 2 Monoclonal Antibody (Catalog # MAB3808) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human AMPK beta 2 (0.5 ng) was included. A specific band for AMPK beta 2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for AMPK beta 2 Antibody (376938)
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line
Sample: A549 human lung carcinoma cell line and MRC-5 human embryonic lung fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: AMPK beta 2
Long Name
AMP-activated Protein Kinase beta 2
Alternate Names
PRKAB2
Gene Symbol
PRKAB2
UniProt
Additional AMPK beta 2 Products
Product Documents for AMPK beta 2 Antibody (376938)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for AMPK beta 2 Antibody (376938)
For research use only
Related Research Areas
Citations for AMPK beta 2 Antibody (376938)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
