Human/Mouse CD27/TNFRSF7 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse CD27/TNFRSF7 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD27/TNFRSF7
    Thr21-Arg182
    Accession # P41272
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse splenocytes
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    3-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse CD27/TNFRSF7 by Western Blot. Western blot shows lysates of human lymph node tissue and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse CD27/TNFRSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD27/TNFRSF7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
CD27/TNFRSF7 in Mouse Splenocytes. CD27/TNFRSF7 was detected in immersion fixed mouse splenocytes using Goat Anti-Human/Mouse CD27/TNFRSF7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF574) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD27/TNFRSF7

CD27 is a lymphocyte-specific member of the tumor necrosis factor receptor superfamily (TNFRSF) and is designated TNFRSF7 (1, 2). Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region (3). Mouse and human CD27 share approximately 65% amino acid identity. CD27 exists as homodimers on the cell surface via an extracellular disulfide bond in the membrane-proximal region. A soluble form of CD27 is also produced during the immune response and is found in various body fluids (4). CD27 is expressed on subsets of T and B cells. The expression of CD27 is upregulated upon T-cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells (5). CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (1, 2). Ligation of CD27 on B cells has been shown to inhibit terminal differentiation of activated mouse B cells into plasma cells and enhances commitment to memory B cell responses (5).

  • References:
    1. Croft, M. (2003) Nature Reviews Immunol. 3:609.
    2. Croft, M. (2003) Cytokine and Growth Factor Reviews 14:265.
    3. Gravestein, L.A. et al. (1993) Eur. J. Immunol. 23:943.
    4. Lens, S.M. et al. (1998) Semin. Immunol. 10:491.
    5. Raman, V.S. et al. (2003) J. Immunol. 171:5876.
  • Entrez Gene IDs:
    939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)
  • Alternate Names:
    CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
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