Human/Mouse Dynactin Subunit 2/DCTN2 Antibody

• Species Reactivity
  Human, Mouse
• Specificity
  Detects human Dynactin Subunit 2/DCTN2 in direct ELISAs and human and mouse Dynactin Subunit 2/DCTN2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DCTN1 is observed.
• Source
  Polyclonal Sheep IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  E. coli-derived recombinant human Dynactin Subunit 2/DCTN2
  Asp277-Lys401
  Accession # Q13561
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• Immunocytochemistry
  5-15 µg/mL
  See below

Western Blot click image to view larger

Detection of Human and Mouse Dynactin Subunit 2/DCTN2 by Western Blot. Western blot shows lysates of human placenta tissue and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dynactin Subunit 2/DCTN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6850) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dynactin Subunit 2/ DCTN2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry click image to view larger

Dynactin Subunit 2/DCTN2 in HeLa Human Cell Line. Dynactin Subunit 2/DCTN2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Dynactin Subunit 2/DCTN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6850) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reconstitution
  Sterile PBS to a final concentration of 0.2 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Entrez Gene IDs:
  10540 (Human); 69654 (Mouse); 299850 (Rat)

• Alternate Names:
  DCTN2; DCTN-50; DCTN5050 kD dynein-associated polypeptide; dynactin 2 (p50); dynactin complex 50 kD subunit; Dynactin complex 50 kDa subunit; Dynactin Subunit 2; dynactin subunit 2,50 kDa dynein-associated polypeptide; Dynamitin; p50 dynamitin; RBP50

FAQs

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