Human/Mouse EBF-2 Antibody

Catalog # Availability Size / Price Qty
AF7006
AF7006-SP
Detection of Human and Mouse EBF-2 by Western Blot.
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Product Details
Citations (3)
FAQs
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Human/Mouse EBF-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse EBF-2 in direct ELISAs. Detects mouse and human EBF-2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) EBF-1 and rmEBF-3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse EBF-2
Arg407-Ser519
Accession # O08792
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse EBF-2 by Western Blot. View Larger

Detection of Human and Mouse EBF-2 by Western Blot. Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EBF-2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry EBF-2 in Mouse Embryo. View Larger

EBF-2 in Mouse Embryo. EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7006) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EBF-2

EBF-2 (Early B cell Factor 2; also Mmot1, OLF3 and COE2) is a 62 kDa (predicted) member of the COE family of transcription factors. It is expressed in immature osteoblasts and Purkinje cells, and in the embryo is associated with the migration of postmitotic neuroblasts. In immature osteoblasts, EBF-2 appears to upregulate OPG, suppressing osteoclast formation. And in the developing retina, EBF-2 is found in ganglion, glycinergic Amacrine and horizontal cells, possibly promoting their development over that of photoreceptor cells. Mouse EBF-2 is 575 amino acids (aa) in length. It contains one DNA-binding region with an embedded C5-type zinc‑finger motif (aa 62-238), a dimerization ITP/TIG domain (aa 253-336), and a Pro/Ser-rich transactivation domain (aa 453-534). Although considered an HLH type transcription factor, it does not contain the typical "b", or basic amino acid sequence associated with bHLH factors. EBF-2 both homodimerizes, and heterodimerizes with EBF-1 and -3. There is an alternative start site at Met23. Over aa 407-519, mouse EBF-2 is identical in aa sequence to rat EBF-2 and shares 99% aa identity with human EBF-2.

Long Name
Early B-cell Factor 2
Entrez Gene IDs
64641 (Human); 13592 (Mouse); 361060 (Rat)
Alternate Names
COE2; EBF2; EBF-2; Mmot1

Product Datasheets

Citations for Human/Mouse EBF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning
    Authors: M Patil, BK Sharma, S Elattar, J Chang, S Kapil, J Yuan, A Satyanaray
    Diabetes, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  2. A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells
    Authors: Silvia Velasco
    Cell Stem Cell, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  3. A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation.
    Authors: Zhao X, Li S, Wang G, Yu Q, Lin J
    Mol Cell, 2014;55(3):372-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP

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Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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