Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Zebrafish

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EBF-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse EBF-3
Ile399-Ser504
Accession # O08791-2

Specificity

Detects endogenous human and mouse EBF-3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse EBF‑3 Antibody

Detection of Human/Mouse EBF‑3 antibody by Western Blot.

Detection of Human/Mouse EBF‑3 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and DA3 mouse myeloma cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EBF-3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse EBF‑3 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and CH-1 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: EBF-3

EBF-3 (Early B cell Factor 3; also OE2 and COE3) is a 65 kDa (predicted) member of the COE family of transcription factors. It has limited expression, being identified principally in cerebellar Purkinje cells, olfactory neurons, and differentiating mantle layer neurons. Mouse EBF-3 is 596 amino acids (aa) in length it contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 371‑431, and a Pro/Ser-rich transactivation domain (aa 464‑555). EBF-3 either homodimerizes, or heterodimerizes with EBF-2 or -1. There are at least two potential splice variants, one that shows a nine aa deletion between aa 252 - 260, and a second that shows the same deletion coupled to a one aa substitution for aa 521‑557. Over aa 399‑504, mouse EBF-3 shows more than 99% aa identity to rat and human EBF-3.

Long Name

Early B-cell Factor 3

Alternate Names

COE3, EBF3, OE-2

Entrez Gene IDs

253738 (Human); 13593 (Mouse); 361668 (Rat)

Gene Symbol

EBF3

UniProt

O08791-2

Additional EBF-3 Products

Product Documents for Human/Mouse EBF‑3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse EBF‑3 Antibody

For research use only

Citations for Human/Mouse EBF‑3 Antibody

Customer Reviews for Human/Mouse EBF‑3 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse EBF‑3 Antibody and earn rewards!

Have you used Human/Mouse EBF‑3 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies