Human/Mouse Frizzled-1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (1)
Supplemental Products

Human/Mouse Frizzled-1 Biotinylated Antibody Summary

Species Reactivity
Human, Mouse
Detects mouse Frizzled-1 in direct ELISAs and Western blots and detects human Frizzled-1 by flow cytometry. In direct ELISAs, approximately 30% cross‑reactivity with recombinant mouse (rm) Frizzled-9 is observed and no cross-reactivity with recombinant human (rh) Frizzled-5, rmFrizzled 2, 3, 4, 6, 7, 8, or rhMFRP is observed.
Monoclonal Rat IgG2A Clone # 162531
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled‑1
Gln1-His248 (Met122Ile)
Accession # O70421
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Frizzled-1 Fc Chimera
Recombinant Mouse Frizzled-1 Fc Chimera (Catalog # 1120-FZ)
Flow Cytometry
0.25 µg/106 cells
HEK293 human embryonic kidney cell line
8-25 µg/mL
Immersion fixed HEK293 human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-1

Frizzled-1 belongs to the frizzled family of 7-transmembrane domain proteins that are receptors for Wnt proteins. Frizzled-1 is expressed in a wide variety of tissues and is involved in cell differentiation, embryonal development and tumorigenesis.

Entrez Gene IDs
8321 (Human); 14362 (Mouse)
Alternate Names
DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor

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Citation for Human/Mouse Frizzled-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Temporal Regulation of Natural Killer T Cell Interferon Gamma Responses by β-Catenin-Dependent and -Independent Wnt Signaling
    Authors: JC Kling, MA Jordan, LA Pitt, J Meiners, T Thanh-Tran, LS Tran, TTK Nguyen, D Mittal, R Villani, RJ Steptoe, K Khosrotehr, SP Berzins, AG Baxter, DI Godfrey, A Blumenthal
    Front Immunol, 2018;9(0):483.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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