hh Human/Mouse HMGB1/HMG-1 Antibody AF1690: R&D Systems

Human/Mouse HMGB1/HMG-1 Antibody

(2 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse HMGB1/HMG‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) HMGB3 is observed, and less than 1% cross-reactivity with rhHMGA1, rhHMGA2, and rhHMGA1b is observed.  
  • Source
    Polyclonal Chicken IgY
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HMGB1/HMG‑1
    Accession # P09429.3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse HMGB1/HMG‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse lactating tissues. PVDF membrane was probed with 1 µg/mL of Chicken Anti-Human/Mouse HMGB1/HMG‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1690) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. A specific band was detected for HMGB1/HMG‑1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HMGB1/HMG-1

Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1-3). Human HMGB1 is expressed as a 25-30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu (4, 5). Residues 27-43 and 178-184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues (6). HMGB1 is expressed at high levels in almost all cells (2, 4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1, 7, 8). It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction (1, 4, 7, 9, 10). The cytokine activity of HBMG1 is restricted to the HMG B box, (3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6, 12). Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4 (1, 3, 4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively.

  • References:
    1. Lotze, M.T. and K.J. Tracey (2005) Nat. Rev. Immunol. 5:331.
    2. Yang, H. et al. (2005) J. Leukoc. Biol. 78:1.
    3. Dumitriu, I.E. et al. (2005) Trends Immunol. 26:381.
    4. Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11.
    5. Wen, L. et al. (1989) Nucleic Acids Res. 17:1197.
    6. Bonaldi, T. et al. (2003) EMBO J. 22:5551.
    7. Muller, S. et al. (2001) EMBO J. 20:4337.
    8. Bustin, M. (1999) Mol. Cell. Biol. 19:5237.
    9. Wang, H. et al. (1999) Science. 285:248.
    10. Dimitriu, I.E. et al. (2005) J. Immunol. 174:7506.
    11. Najima, Y. et al. (2005) J. Biol. Chem. 280:27523.
    12. Gardella, S. et al. (2002) EMBO Rep. 3:995.
  • Long Name:
    High Mobility Group Protein 1
  • Entrez Gene IDs:
    3146 (Human)
  • Alternate Names:
    Amphoterin; high mobility group box 1; High mobility group protein 1; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG1; HMG-1; HMG1DKFZp686A04236; HMG3; HMGB1; SBP-1; Sulfoglucuronyl carbohydrate binding protein
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Sample Type
  1. Cancer usurps skeletal muscle as an energy repository.
    Authors: Luo, Yi, Yoneda, Junya, Ohmori, Hitoshi, Sasaki, Takamits, Shimbo, Kazutaka, Eto, Sachise, Kato, Yumiko, Miyano, Hiroshi, Kobayashi, Tsuyoshi, Sasahira, Tomonori, Chihara, Yoshitom, Kuniyasu, Hiroki
    Cancer Res, 2014;74(1):330-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis.
    Authors: Liou T, Adler F, Keogh R, Li Y, Jensen J, Walsh W, Packer K, Clark T, Carveth H, Chen J, Rogers S, Lane C, Moore J, Sturrock A, Paine R, Cox D, Hoidal J
    PLoS ONE, 2012;7(8):e42748.
    Species: Human
    Sample Type: Sputum
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Normal Chicken IgY Control

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