Detection of Human/Mouse HO‑1/HMOX1/HSP32 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO‑1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO‑1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme Oxygenase occurs as 2 isozymes, an inducible Heme Oxygenase-1 (HO-1/HMOX1) and a constitutive Heme Oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.
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