Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 40007
View all IL-1ra/IL-1F3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-1ra
Arg27-Gln178
Accession # P25085

Specificity

Detects human and mouse IL-1ra in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse IL‑1ra/IL‑1F3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA)
Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB480 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1ra/IL-1F3

IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Long Name

Interleukin 1 Receptor Antagonist

Alternate Names

DIRA, ICIL-1ra, IL-1F3, IL-1ra3, IL-1RN, IL1ra, IL1RN, MVCD4

Entrez Gene IDs

3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine); 100034236 (Equine)

Gene Symbol

IL1RN

UniProt

P25085

Additional IL-1ra/IL-1F3 Products

Product Documents for Human/Mouse IL‑1ra/IL‑1F3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse IL‑1ra/IL‑1F3 Antibody

For research use only

Citations for Human/Mouse IL‑1ra/IL‑1F3 Antibody

Customer Reviews for Human/Mouse IL‑1ra/IL‑1F3 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
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1 Stars
0%

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  • Human/Mouse IL-1ra/IL-1F3 Antibody
    Name: Alisha Freeman
    Application: ELISA
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 04/05/2018
  • Human/Mouse IL-1ra/IL-1F3 Antibody
    Name: Alisha Freeman
    Application: ELISA
    Sample Tested: Differentiated rat mesenchymal stem cells
    Species: Rat
    Verified Customer | Posted 10/31/2017

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FAQs

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Associated Pathways

IL-1 Family Signaling Pathways IL-1 Family Signaling Pathway Thumbnail