Detection of Human Jumonji/JARID2 by Western Blot. |
Western blot shows lysates of IMR‑32 human neuroblastoma cell line and Neuro‑2A mouse neuroblastoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse Jumonji/JARID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6090) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Jumonji/JARID2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Jumonji [Japanese for "cruciform/cross" also JARID2 (Jumonji/ARID domain‑containing protein 2) and JMJ] is a 140‑155 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. Jumonji is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human Jumonji is 1246 amino acids (aa) in length. It contains an NLS (aa 1‑131), a transcriptional repressor domain (aa 132‑222), a DNA binding region (aa 558‑709) and a nonfunctional histone demethylase domain (aa 916‑1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304‑1151, and a third contains a 22 aa substitution for aa 1‑60, coupled to a 50 aa substitution for aa 949‑1246. Over aa 1‑159, human Jumonji shares 96% aa identity with mouse Jumonji.
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