Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunoprecipitation, CyTof

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Jumonji/JARID2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Jumonji/JARID2
Met1-Thr159
Accession # Q92833

Specificity

Detects human and mouse Jumonji/JARID2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human JARID2B is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse Jumonji/JARID2 Antibody

Detection of Human Jumonji/JARID2 antibody by Western Blot.

Detection of Human Jumonji/JARID2 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse Jumonji/JARID2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6090) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Jumonji/JARID2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human/Mouse Jumonji/JARID2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and Neuro‑2A mouse neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jumonji/JARID2

Jumonji [Japanese for "cruciform/cross" also JARID2 (Jumonji/ARID domain‑containing protein 2) and JMJ] is a 140‑155 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. Jumonji is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human Jumonji is 1246 amino acids (aa) in length. It contains an NLS (aa 1‑131), a transcriptional repressor domain (aa 132‑222), a DNA binding region (aa 558‑709) and a nonfunctional histone demethylase domain (aa 916‑1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304‑1151, and a third contains a 22 aa substitution for aa 1‑60, coupled to a 50 aa substitution for aa 949‑1246. Over aa 1‑159, human Jumonji shares 96% aa identity with mouse Jumonji.

Alternate Names

JARID2, JMJ

Entrez Gene IDs

3720 (Human); 16468 (Mouse)

Gene Symbol

JARID2

UniProt

Q92833 (Human)

Additional Jumonji/JARID2 Products

Product Documents for Human/Mouse Jumonji/JARID2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Jumonji/JARID2 Antibody

For research use only

Related Research Areas

Epigenetics

Citations for Human/Mouse Jumonji/JARID2 Antibody

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