Human/Mouse Lysine (K)-specific Demethylase 4A/KDM4A Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human KDM4A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4C is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human KDM4A
    Lys386-Ser514
    Accession # AAH02558
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Lysine (K)‑specific Demethylase 4A/KDM4A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, THP‑1 human acute monocytic leukemia cell line, and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Human Lysine (K)‑specific Demethylase 4A/KDM4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6434) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysine (K)‑specific Demethylase 4A/KDM4A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunocytochemistry
KDM4A in BG01V Human Cell Line. KDM4A was detected in immersion fixed endoderm-differentiated BG01V human embryonic stem cells using Human/Mouse KDM4A Affinity-purified Polyclonal Antibody (Catalog # AF6434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lysine (K)-specific Demethylase 4A/KDM4A
KDM4A (Lysine [K]-specific demethylase 4A; also known as JMJD2A) is a 155-160 kDa member of the JHDM3 histone demethylase family of enzymes. It is ubiquitously expressed and interacts with the histone deacetylase proteins HDAC-1, -2, and -3. KDM4A specifically demethylates trimethylated lysines on histone H3 at Lys9 and Lys36. Transcriptional repression occurs when it interacts with NCOR1. Human KDM4A is 1064 amino acids (aa) in length (SwissProt #:O75164). It contains one jumonji N domain (aa 13-55), a catalytic jumonji C domain (aa 175-291), two PHD zinc finger regions (aa 709-855), and two TUDOR methylated-histone binding domains (aa 897-1011). Over aa 386-514, human KDM4A shares 93% amino acid sequence identity with mouse KDM4A.
  • Entrez Gene IDs:
    9682 (Human); 230674 (Mouse); 313539 (Rat)
  • Alternate Names:
    EC 1.14.11; EC 1.14.11.-; JHDM3A; JHDM3AJMJD2; JmjC domain-containing histone demethylation protein 3A; JMJD2; JMJD2A; JMJD2Alysine-specific demethylase 4A; jumonji domain containing 2; jumonji domain containing 2A; Jumonji domain-containing protein 2A; KDM4A; KIAA0677jumonji C domain-containing histone demethylase 3A; Lysine (K)specific Demethylase 4A; Lysine (K)-specific Demethylase 4A
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