Human/Mouse PDZK1 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse PDZK1.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PDZK1
    Met1-Met519
    Accession # Q5T2W1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
PDZK1 in Human Kidney. PDZK1 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human/Mouse PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4997) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of proximal tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human PDZK1 by Western Blot. Western blot shows lysates of Calu‑6 human lung carcinoma cell line, U266 human myeloma cell line, MCF‑7 human breast cancer cell line, and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4997) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PDZK1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDZK1

PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70 kDa/CAP70) is a 65 - 70 kDa member of the NHER (Na/H Exchange Regulator) family of proteins. It is only expressed in epithelium, particularly that found in intestine, kidney and liver. PDZK1 serves as a scaffold protein that connects membrane proteins to cytoplasmic regulators. Human PDZK1 is 519 amino acids (aa) in length. It contains four PDZ domains (aa 9 - 90; 134 - 215; 243 - 323; and 378 - 458) plus two phosphorylation sites at Ser492 and Ser514. There are two potential truncated forms of human PDZK1, one that ends after Leu330, and a second that ends after Lys264. Human PDZK1 shares 78% aa identity with mouse PDZK1.

  • Long Name:
    PDZ Domain Containing 1
  • Entrez Gene IDs:
    5174 (Human); 59020 (Mouse); 65144 (Rat)
  • Alternate Names:
    CAP70; CFTR-associated protein of 70 kDa; CLAMP; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; NHERF3; NHERF-3; NHERF3PDZD1Na(+)/H(+) exchange regulatory cofactor NHE-RF3; PDZ domain containing 1; PDZ domain-containing protein 1; PDZ-containing kidney protein 1; PDZD1; PDZK1; Sodium-hydrogen exchanger regulatory factor 3
Related Research Areas
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Isotype Controls
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Secondary Antibodies
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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