PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70 kDa/CAP70) is a 65 - 70 kDa member of the NHER (Na/H Exchange Regulator) family of proteins. It is only expressed in epithelium, particularly that found in intestine, kidney and liver. PDZK1 serves as a scaffold protein that connects membrane proteins to cytoplasmic regulators. Human PDZK1 is 519 amino acids (aa) in length. It contains four PDZ domains (aa 9 - 90; 134 - 215; 243 - 323; and 378 - 458) plus two phosphorylation sites at Ser492 and Ser514. There are two potential truncated forms of human PDZK1, one that ends after Leu330, and a second that ends after Lys264. Human PDZK1 shares 78% aa identity with mouse PDZK1.
Human/Mouse PDZK1 Antibody
R&D Systems | Catalog # AF4997
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human PDZK1
Met1-Met519
Accession # Q5T2W1
Met1-Met519
Accession # Q5T2W1
Specificity
Detects human and mouse PDZK1.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse PDZK1 Antibody
Detection of Human PDZK1 by Western Blot.
Western blot shows lysates of Calu-6 human lung carcinoma cell line, U266 human myeloma cell line, MCF-7 human breast cancer cell line, and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4997) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PDZK1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDZK1 in Human Kidney.
PDZK1 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human/Mouse PDZK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4997) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of proximal tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse PDZK1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and pancreas
Sample: Immersion fixed paraffin-embedded sections of human kidney and pancreas
Western Blot
1 µg/mL
Sample: Calu-6 human lung carcinoma cell line, U266 human myeloma cell line, MCF-7 human breast cancer cell line, and mouse liver tissue
Sample: Calu-6 human lung carcinoma cell line, U266 human myeloma cell line, MCF-7 human breast cancer cell line, and mouse liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PDZK1
Long Name
PDZ Domain Containing 1
Alternate Names
CAP70, CLAMP, NaPi-Cap1, NHERF3, PDZD1
Gene Symbol
PDZK1
UniProt
Additional PDZK1 Products
Product Documents for Human/Mouse PDZK1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse PDZK1 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse PDZK1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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