Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PGPEP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PGPEP-1
Met1-His209
Accession # Q9NXJ5

Specificity

Detects human PGPEP-1 in direct ELISAs and, human and mouse PGPEP-1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse PGPEP‑1 Antibody

Detection of Human and Mouse PGPEP-1 antibody by Western Blot.

Detection of Human and Mouse PGPEP‑1 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line, JAR human choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PGPEP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6278) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PGPEP-1 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse PGPEP‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line, JAR human choriocarcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and human brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PGPEP-1

Pyroglutamyl peptidase-1 (PGPEP-1) is an omega peptidase which removes pyroglutamyl residues from the amino termini of peptides and proteins (1). It is a cytosolic cysteine peptidase that is expressed in most cell types (2). The enzyme requires a thiol‑reducing agent for activity (3). PGPEP-1 is potentially involved in the inactivation of biologically active peptides that possess an amino terminal pyroglutamyl group (3). Examples of such peptides include neurotensin, luteinizing hormone releasing hormone, and thyrotropin-releasing hormone. Human and mouse PGPEP-1 share 95% aa sequence identity.

References

  1. Kilbane Z. et al. (2007) Mol. Cell. Biochem. 297:189.
  2. Cummins P.M. and B. O'Connor (1998) Biochim. Biophys. Acta. 1429:1.
  3. Dando P.M. et al. (2003) Protein Express. Purif. 28:111.

Long Name

Pyroglutamyl Aminopeptidase I

Alternate Names

PAP-I, PGPEP1

Entrez Gene IDs

54858 (Human)

Gene Symbol

PGPEP1

UniProt

Q9NXJ5

Additional PGPEP-1 Products

Product Documents for Human/Mouse PGPEP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse PGPEP‑1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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