Human/Mouse R-Spondin 3 Antibody
Human/Mouse R-Spondin 3 Antibody Summary
Accession # BAC36296
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: R-Spondin 3
R-Spondin 3 (RSPO3, roof plate-specific spondin 3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is an ~31 kDa secreted protein that shares ~40% amino acid (aa) identity with the other three R-Spondin family members (1, 2). All are positive modulators of Wnt/ beta -catenin signaling, but each has a distinct expression pattern (1‑4). Like other R-spondins, R-Spondin 3 contains two adjacent cysteine-rich furin-like domains (aa 35‑135) with one potential N-glycosylation site (aa 36), followed by a thrombospondin (TSP-1) motif (aa 147‑207) and a region rich in basic residues (aa 211‑269). Only the furin-like domains are needed for beta -catenin stabilization (2). Within aa 21‑209, human R-Spondin 3 shares 93%, 92%, 97%, 96% and 92% aa identity with mouse, rat, equine, bovine and canine R-Spondin 3, respectively. Potential isoforms of 279 and 297 aa diverge at aa 210 and 276, respectively (5). Mouse R-Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta‑specific transcription factor, Gcm1. In the mouse embryo, R-Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (8‑10). R-Spondin 3 has also been identified as an oncogene (11).
- Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
- Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
- Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
- Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
- Entrez Accession # EAW48114 and EAW48116.
- Kazanskaya, O. et al. (2008) Development 135:3655.
- Aoki, M. et al. (2007) Dev. Biol. 301:218.
- Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
- Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
- Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
- Theodorou, V. et al. (2007) Nat. Genet. 6:759.
Citation for Human/Mouse R-Spondin 3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Nkx2-5 regulates cardiac growth through modulation of Wnt signaling by R-spondin3.
Authors: Cambier L, Plate M, Sucov H, Pashmforoush M
Sample Types: Whole Tissue
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