Human/Mouse/Rat AMPK beta 1 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF2854V Datasheet / COA / SDS

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AF2854V-100UG

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All AMPK beta 1 Antibodies

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Human/Mouse/Rat AMPK beta 1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat AMPK beta 1 in Western blots. In Western blots, no cross-reactivity with recombinant human AMPK beta 2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human AMPKÎ²1
Met1-Ile270
Accession # Q9Y478

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AMPK beta 1

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2 subunit in liver, while beta 2 is more abundant than beta 1 in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase complex as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase beta 1

Entrez Gene IDs

5564 (Human); 19079 (Mouse); 83803 (Rat)

Alternate Names

5'-AMP-activated protein kinase beta-1 subunit; AMP-activated protein kinase beta subunit; AMPK beta -1 chain; AMPK beta 1; AMPK beta 1,5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPK; AMPKb; HAMPKb; MGC17785; PRKAB1; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1