Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AMPK beta 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AMPK beta 1
Met1-Ile270
Accession # Q9Y478

Specificity

Detects human, mouse, and rat AMPK beta 1 in Western blots. In Western blots, no cross-reactivity with recombinant human AMPK beta 2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat AMPK beta 1 Antibody

Detection of Human/Mouse/Rat AMPK beta 1 antibody by Western Blot.

Detection of Human/Mouse/Rat AMPK beta 1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human AMPK beta 1 and AMPK beta 2 (2 ng/lane) were included. A specific band for AMPK beta 1 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of AMPK beta 1 in Human Kidney.

AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of AMPK beta 1 in Mouse Kidney.

AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Mouse Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of AMPK beta 1 in Rat Kidney.

AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Rat Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat AMPK beta 1 Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Kidney, Mouse Kidney and Rat Kidney

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line

Reviewed Applications

Read 1 review rated 4 using AF2854 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AMPK beta 1

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2 subunit in liver, while beta 2 is more abundant than beta 1 in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase complex as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase beta 1

Alternate Names

PRKAB1

Entrez Gene IDs

5564 (Human); 19079 (Mouse); 83803 (Rat)

Gene Symbol

PRKAB1

UniProt

Q9Y478

Additional AMPK beta 1 Products

Product Documents for Human/Mouse/Rat AMPK beta 1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat AMPK beta 1 Antibody

For research use only

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

Citations for Human/Mouse/Rat AMPK beta 1 Antibody

Customer Reviews for Human/Mouse/Rat AMPK beta 1 Antibody (1)

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  • Human/Mouse/Rat AMPK beta 1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipose tissue
    Species: Mouse
    Verified Customer | Posted 07/19/2019
    Human/Mouse/Rat AMPK beta 1 Antibody AF2854

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Protocols

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FAQs

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