Human/Mouse/Rat AMPK beta 1 Antibody Summary
Met1-Ile270
Accession # Q9Y478
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat AMPK beta 1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human AMPK beta 1 and AMPK beta 2 (2 ng/lane) were included. A specific band for AMPK beta 1 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of AMPK beta 1 in Human Kidney. AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of AMPK beta 1 in Mouse Kidney. AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Mouse Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of AMPK beta 1 in Rat Kidney. AMPK beta 1 was detected in immersion fixed paraffin-embedded sections of Rat Kidney using Goat Anti-Human/Mouse/Rat AMPK beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2854) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AMPK beta 1
AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2 subunit in liver, while beta 2 is more abundant than beta 1 in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase complex as a therapeutic target in heart disease, obesity, and diabetes.
Product Datasheets
Citations for Human/Mouse/Rat AMPK beta 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Effects of genetic deletion of soluble 5′-nucleotidases NT5C1A and NT5C2 on AMPK activation and nucleotide levels in contracting mouse skeletal muscles
Authors: Samanta Kviklyte, Didier Vertommen, Xavier Yerna, Harriet Andersén, Xiufeng Xu, Philippe Gailly et al.
American Journal of Physiology-Endocrinology and Metabolism
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AMPK antagonizes hepatic glucagon-stimulated cyclic AMP signalling via phosphorylation-induced activation of cyclic nucleotide phosphodiesterase 4B
Authors: M. Johanns, Y.-C. Lai, M.-F. Hsu, R. Jacobs, D. Vertommen, J. Van Sande et al.
Nature Communications
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