|Detection of Bcl‑xL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, C6 rat glioma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl‑xL Monoclonal Antibody (Catalog # MAB894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl‑xL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|Detection of Human Bcl‑xL by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑xL at approximately 33 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl‑xL Monoclonal Antibody (Catalog # MAB894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.|
Bcl-xL is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-xL is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-xL is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-xL contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-xL missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-xL appears to be mediated through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
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