Human/Mouse/Rat Bcl-xL Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB894-SP
MAB894-100
Human/Mouse/Rat Bcl-xL Antibody in Data
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Product Details
Citations (1)
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Human/Mouse/Rat Bcl-xL Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Bcl‑xL in direct ELISAs and detects human, mouse, and rat Bcl‑xL in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2052A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human Bcl‑xL
Met1-Arg212
Accession # Q07817
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Bcl‑x<sub>L</sub>by Western Blot. View Larger

Detection of Bcl‑xLby Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, C6 rat glioma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl-xLMonoclonal Antibody (Catalog # MAB894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-xLat approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Bcl‑x<sub>L</sub>by Simple Western<sup>TM</sup>. View Larger

Detection of Human Bcl‑xLby Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑xLat approximately 33 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl‑xLMonoclonal Antibody (Catalog # MAB894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-xL

Bcl-xL is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-xL is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-xL is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-xL contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-xL missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-xL appears to be mediated through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

References
  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
Long Name
B Cell Lymphoma/Leukemia x Long Form
Entrez Gene IDs
598 (Human); 12048 (Mouse); 24888 (Rat)
Alternate Names
Apoptosis regulator Bcl-X; bcl2-L-1; BCL2-like 1; bcl-2-like protein 1; BCLXBCL2LBcl-X; BclxL; Bcl-xL; BCL-XL/S; BCLXS; bcl-xS; DKFZp781P2092

Product Datasheets

Citation for Human/Mouse/Rat Bcl-xL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The novel TRAIL-receptor agonist APG350 exerts superior therapeutic activity in pancreatic cancer cells
    Authors: K Legler, C Hauser, JH Egberts, A Willms, C Heneweer, S Boretius, C Röcken, CC Glüer, T Becker, M Kluge, O Hill, C Gieffers, H Fricke, H Kalthoff, J Lemke, A Trauzold
    Cell Death Dis, 2018;9(5):445.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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