Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2052A
View all Bcl-xL Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bcl‑xL
Met1-Arg212
Accession # Q07817

Specificity

Detects human Bcl‑xL in direct ELISAs and detects human, mouse, and rat Bcl‑xL in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Bcl‑xL Antibody

Detection of Bcl‑xLby Western Blot.

Detection of Bcl‑xLby Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, C6 rat glioma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl-xLMonoclonal Antibody (Catalog # MAB894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Bcl-xLat approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Bcl‑xLby Simple WesternTM.

Detection of Human Bcl‑xLby Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑xLat approximately 33 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl‑xLMonoclonal Antibody (Catalog # MAB894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Bcl‑xL in Human Prostate.

Bcl‑xL was detected in immersion fixed paraffin-embedded sections of Human Prostate using Rabbit Anti-Human/Mouse/Rat Bcl‑xL Monoclonal Antibody (Catalog # MAB894) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to secretetory vesicles in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat Bcl‑xL Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, C6 rat glioma cell line, and RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bcl-xL

Bcl-xL is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-xL is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-xL is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-xL contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-xL missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-xL appears to be mediated through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

References

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.

Long Name

B Cell Lymphoma/Leukemia x Long Form

Alternate Names

BclxL

Entrez Gene IDs

598 (Human); 12048 (Mouse); 24888 (Rat)

Gene Symbol

BCL2L1

UniProt

Q07817

Additional Bcl-xL Products

Product Documents for Human/Mouse/Rat Bcl‑xL Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Bcl‑xL Antibody

For research use only

Citations for Human/Mouse/Rat Bcl‑xL Antibody

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Protocols

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