Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2204C
View all beta-2 Adrenergic R/ADRB2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human beta ‑2 Adrenergic R/ADRB2
Accession # P07550

Specificity

Detects human beta ‑2 Adrenergic R/ADRB2 in direct ELISAs. Detects human, mouse, and rat beta ‑2 Adrenergic R/ADRB2 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody

Detection of Human, Mouse, and Rat beta -2 Adrenergic R/ADRB2 antibody by Western Blot.

Detection of Human, Mouse, and Rat beta ‑2 Adrenergic R/ADRB2 by Western Blot.

Western blot shows lysates of human heart tissue, mouse colon tissue, and rat colon tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat beta -2 Adrenergic R/ADRB2 Monoclonal Antibody (Catalog # MAB10040) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for beta -2 Adrenergic R/ADRB2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human heart tissue, Mouse colon tissue, and Rat colon tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-2 Adrenergic R/ADRB2

Beta-2 adrenoceptor (ADRB2) is a 413 amino acids adrenergic receptor expressed in smooth muscle and metabolic tissues. Beta-adrenergic receptors belong to the seven-transmembrane domain/GPCR family of receptors and mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Activation of ADRB2 induces a decrease in gastrointestinal motility, bronchodilation, vasodilation in skeletal and cardiac muscle, and glycogenolysis in liver. Agonists of ADRB2 are most widely used for the treatment of asthma. In complex with beta-arrestin-1 and c-src, the beta-2 adrenergic receptor activates MAP kinases ERK1(MAPK3) and ERK2 (MAPK1). Expression of the beta-2 adrenoceptor has been reported in adipose, blood, brain, heart, lung, nose, pancreas, skeletal muscle, skin, and vessel.

Long Name

Beta-2 Adrenergic Receptor

Alternate Names

ADRB2, ADRB2R, B2AR, beta-2 AdrenergicR, beta2 AdrenergicR

Entrez Gene IDs

154 (Human); 11555 (Mouse); 24176 (Rat)

Gene Symbol

ADRB2

UniProt

P07550

Additional beta-2 Adrenergic R/ADRB2 Products

Product Documents for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody

For research use only

Citations for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody

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