Human/Mouse/Rat beta -2 Adrenergic R/ADRB2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10040-100
MAB10040-SP
Detection  of Human, Mouse, and Rat  beta ‑2 Adrenergic R/ADRB2 by Western Blot.
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Human/Mouse/Rat beta -2 Adrenergic R/ADRB2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human beta ‑2 Adrenergic R/ADRB2 in direct ELISAs. Detects human, mouse, and rat beta ‑2 Adrenergic R/ADRB2 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2204C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human beta ‑2 Adrenergic R/ADRB2
Accession # P07550
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human, Mouse, and Rat  beta ‑2 Adrenergic R/ADRB2 by Western Blot. View Larger

Detection of Human, Mouse, and Rat beta ‑2 Adrenergic R/ADRB2 by Western Blot. Western blot shows lysates of human heart tissue, mouse colon tissue, and rat colon tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat beta ‑2 Adrenergic R/ADRB2 Monoclonal Antibody (Catalog # MAB10040) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for beta ‑2 Adrenergic R/ADRB2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-2 Adrenergic R/ADRB2

Beta-2 adrenoceptor (ADRB2) is a 413 amino acids adrenergic receptor expressed in smooth muscle and metabolic tissues. Beta-adrenergic receptors belong to the seven-transmembrane domain/GPCR family of receptors and mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Activation of ADRB2 induces a decrease in gastrointestinal motility, bronchodilation, vasodilation in skeletal and cardiac muscle, and glycogenolysis in liver. Agonists of ADRB2 are most widely used for the treatment of asthma. In complex with beta-arrestin-1 and c-src, the beta-2 adrenergic receptor activates MAP kinases ERK1(MAPK3) and ERK2 (MAPK1). Expression of the beta-2 adrenoceptor has been reported in adipose, blood, brain, heart, lung, nose, pancreas, skeletal muscle, skin, and vessel.

Long Name
Beta-2 Adrenergic Receptor
Entrez Gene IDs
154 (Human); 11555 (Mouse); 24176 (Rat)
Alternate Names
ADRB2; ADRB2R; ADRBR; adrenergic, beta-2-, receptor, surface; B2AR; B2ARADRB2R; BAR; beta-2 Adrenergic R; beta-2 adrenergic receptor; beta2 AdrenergicR; beta-2 AdrenergicR; Beta-2 adrenoceptor; Beta-2 adrenoreceptor; BETA2AR; catecholamine receptor

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