Human/Mouse/Rat Calcineurin A Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat Calcineurin A by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat Calcineurin A Monoclonal Antibody (Catalog # MAB2839) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Calcineurin (Catalog # 3160-CA) (1 ng) was included. A specific band for Calcineurin A was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calcineurin A
Calcineurin A, also known as PP2B and PPP3CA, is the 59 kDa catalytic subunit of the calcium/calmodulin-dependent protein phosphatase. When activated by calcium in the presence of the regulatory B subunit and calmodulin, Calcineurin A selectively removes phosphates from serine and threonine residues on target proteins. Although ubiquitously expressed, Calcineurin levels are highest in brain, where the phosphatase plays a role in the formation and retention of memories. Calcineurin activity is inhibited by the immunosuppressant drug cyclosporine A bound to cyclophilins.
Citation for Human/Mouse/Rat Calcineurin A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Insulin dysfunction induces in vivo tau hyperphosphorylation through distinct mechanisms.
Authors: Planel E, Tatebayashi Y, Miyasaka T, Liu L, Wang L, Herman M, Yu WH, Luchsinger JA, Wadzinski B, Duff KE, Takashima A
J. Neurosci., 2007;27(50):13635-48.
Sample Types: Cell Lysates
Applications: Western Blot
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