Calcineurin A, also known as PP2B and PPP3CA, is the 59 kDa catalytic subunit of the calcium/calmodulin-dependent protein phosphatase. When activated by calcium in the presence of the regulatory B subunit and calmodulin, Calcineurin A selectively removes phosphates from serine and threonine residues on target proteins. Although ubiquitously expressed, Calcineurin levels are highest in brain, where the phosphatase plays a role in the formation and retention of memories. Calcineurin activity is inhibited by the immunosuppressant drug cyclosporine A bound to cyclophilins.
Calcineurin A Antibody (302202)
R&D Systems | Catalog # MAB2839
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 302202
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Product Specifications
Immunogen
Bovine brain Calcineurin holoenzyme (A and B subunits)
Specificity
Detects human, mouse, and rat Calcineurin A.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Calcineurin A Antibody (302202)
Detection of Human/Mouse/Rat Calcineurin A by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat Calcineurin A Monoclonal Antibody (Catalog # MAB2839) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Calcineurin (Catalog # 3160-CA) (1 ng) was included. A specific band for Calcineurin A was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Calcineurin A Antibody (302202)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line
Sample: HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Calcineurin A
Alternate Names
CALNA, CNA1, PP2B, PP3CA, PPP2B, PPP3CA
Gene Symbol
PPP3CA
Additional Calcineurin A Products
Product Documents for Calcineurin A Antibody (302202)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Calcineurin A Antibody (302202)
For research use only
Citations for Calcineurin A Antibody (302202)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
