Detection of Human, Mouse, and Rat Carbonic Anhydrase III/CA3 by Western Blot. Western blot shows lysates of human, mouse, and rat skeletal muscle tissue and heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/|
Rat Carbonic Anhydrase III/CA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2185) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase III/CA3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1).
Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2).
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