Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Casein Kinase 1 epsilon Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CK1 epsilon
Met317-Lys416
Accession # P49674

Specificity

Detects endogenous human, mouse and rat CK1 epsilon in Western blots. In Western blots, this antibody does not cross‑react with recombinant human CK1 alpha, CK1 delta, or CK1 gamma.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Casein Kinase 1 epsilon Antibody

Detection of Human/Mouse/Rat Casein Kinase 1e antibody by Western Blot.

Detection of Human/Mouse/Rat Casein Kinase 1 epsilon by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4567) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1 epsilon at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Casein Kinase 1 epsilon Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NRK rat normal kidney cell, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line, PC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Casein Kinase 1 epsilon

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3 , delta  and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 epsilon phosphorylates all three mammalian PER proteins, as well as BMAL1 and CRY, thereby modulating the activities of these circadian clock regulators.

Alternate Names

CSNK1E

Entrez Gene IDs

1454 (Human); 27373 (Mouse); 58822 (Rat)

Gene Symbol

CSNK1E

UniProt

P49674

Additional Casein Kinase 1 epsilon Products

Product Documents for Human/Mouse/Rat Casein Kinase 1 epsilon Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Casein Kinase 1 epsilon Antibody

For research use only

Related Research Areas

Intracellular Kinases

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