|Detection of Human/Mouse/Rat Casein Kinase 1 epsilon by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1 epsilon Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4567) followed by HRP‑conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1 epsilon at approximately 47 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.|
Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, gamma 1, gamma 2, gamma 3 ,δ and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1 epsilon phosphorylates all three mammalian PER proteins, as well as BMAL1 and CRY, thereby modulating the activities of these circadian clock regulators.
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