Detection of Human, Mouse, and Rat Casein Kinase 2 beta /CK2 beta by Western Blot. Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line, Neuro‑2A mouse neuroblastoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Casein Kinase 2 beta /CK2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4380) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 2 beta /CK2 beta at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Casein Kinase 2 beta in Neuro‑2A Mouse Cell Line. Casein Kinase 2 beta was detected in immersion fixed Neuro‑2A mouse neuroblastoma cell line using Sheep Anti-Human/Mouse/Rat Casein Kinase 2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4380) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Casein Kinase 2 beta
Casein kinase 2 (CK2) is a ubiquitous, tetrameric serine/threonine kinase comprised of two catalytic subunits (CK2 alpha and/or CK2 alpha ‘) and two identical regulatory subunits (CK2 beta ). CK2 has been implicated in numerous cellular processes, including signal transduction, transcription, translation, replication, and metabolic pathways. CK2 beta undergoes phosphorylation at Ser-2, Ser-3 and Ser-209. CK2 beta also plays dual roles in the regulation of CK2 acivity. The C-terminal domain is responsible for stable interactions with the catalytic subunit and increased catalytic activity following tetramer formation, while the N-terminal domain exerts negative regulation on the catalytic activity of CK2. CK2 is known to phosphorylate more than 300 different substrates. Phosphorylation of cell-cycle proteins such as p53, p34cdc2, p27KIP1, and MDM-2 account for the ability of CK2 to induce proliferation, while the phosphorylation of HS1, Bid, and Max account for its antiapoptotic role.
Entrez Gene IDs:
1460 (Human); 13001 (Mouse); 81650 (Rat)
casein kinase 2, beta polypeptide; Casein kinase II beta subunit; casein kinase II subunit beta; CK II beta; CK2B; CK2N; CSK2B; CSNK2B; G5A; MGC138222; MGC138224; Phosvitin; Protein G5a
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