|Detection of Human/Mouse/Rat CSE1L/CAS by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CSE1L/CAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CSE1L/CAS at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
CSE1L (Chromosome segregation 1-like protein; also known as Exportin-2 (XPO2) and Cellular Apoptosis Susceptibility (CAS)) is a 110 kDa member of the XPO2/CSE1 family of proteins. It is expressed in divergent proliferating cell types such as colon and mammary epithelium, fibroblasts, hepatocytes, and spermatogonia. It participates in the export of importin-alpha from the nucleus, and regulates p53-sensitive gene transcription. Human CSE1L is 971 amino acids (aa) in length and contains an N-terminal importin domain (aa 29‑102) and a DNA-association region (aa 372‑385). There are multiple splice variants. There is a six aa substitution for aa 190‑971, separate deletions of aa 133‑341 and 257‑312, an alternate start site at Met235 with a deletion of aa 350‑517, and an alternate start site at Met100 with a 25 aa substitution for aa 674 - 971. Over aa 739‑933, human CSE1L shows 100% aa identity to mouse CSE1L.
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