Human/Mouse/Rat CSE1L/CAS Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human, mouse, and rat CSE1L in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CSE1L
    Ala739-His933
    Accession # P55060
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat CSE1L/CAS by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CSE1L/CAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CSE1L/CAS at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CSE1L/CAS

CSE1L (Chromosome segregation 1-like protein; also known as Exportin-2 (XPO2) and Cellular Apoptosis Susceptibility (CAS)) is a 110 kDa member of the XPO2/CSE1 family of proteins. It is expressed in divergent proliferating cell types such as colon and mammary epithelium, fibroblasts, hepatocytes, and spermatogonia. It participates in the export of importin-alpha from the nucleus, and regulates p53-sensitive gene transcription. Human CSE1L is 971 amino acids (aa) in length and contains an N-terminal importin domain (aa 29‑102) and a DNA-association region (aa 372‑385). There are multiple splice variants. There is a six aa substitution for aa 190‑971, separate deletions of aa 133‑341 and 257‑312, an alternate start site at Met235 with a deletion of aa 350‑517, and an alternate start site at Met100 with a 25 aa substitution for aa 674 - 971. Over aa 739‑933, human CSE1L shows 100% aa identity to mouse CSE1L.

  • Long Name:
    Chromosome segregation 1-like protein
  • Entrez Gene IDs:
    1434 (Human); 110750 (Mouse); 362273 (Rat)
  • Alternate Names:
    CAS; CASMGC117283; Cellular apoptosis susceptibility protein; chromosome segregation 1 (yeast homolog)-like; Chromosome segregation 1-like protein; CSE1 chromosome segregation 1-like (yeast); CSE1; CSE1L; exp2; exportin-2; Importin-alpha re-exporter; MGC130037; XPO2; XPO2MGC130036
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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