Human/Mouse/Rat Ephrin-B2 Antibody

Catalog # Availability Size / Price Qty
AF496
AF496-SP
Detection of Mouse Ephrin‑B2 by Western Blot.
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Product Details
Citations (26)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat Ephrin-B2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Ephrin-B1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B2
Arg27-Ala227
Accession # AAA82934
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Ephrin‑B2 antibody by Western Blot. View Larger

Detection of Mouse Ephrin‑B2 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ephrin-B2 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of Ephrin-B2 antibody in SH-SY5Y Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Ephrin‑B2 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry Ephrin-B2 antibody in Rat Hippo-campal Neurons by Immunocytochemistry (ICC). View Larger

Ephrin‑B2 in Rat Hippo-campal Neurons. Ephrin‑B2 was detected in immersion fixed rat hippocampal neurons using 2 µg/mL Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-B2

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of murine and human Ephrin-B2 share 98% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Entrez Gene IDs
1948 (Human); 13642 (Mouse); 30219 (Zebrafish)
Alternate Names
EFNB2; ELF-2; ephrin B2; EphrinB2; Ephrin-B2; EPLG5; HTKL; Htk-L; LERK5; LERK-5; NLERK-1

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Citations for Human/Mouse/Rat Ephrin-B2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Histochemical examination of blood vessels in murine femora with intermittent PTH administration
    Authors: H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa
    Journal of oral biosciences, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture
    Authors: S Yamasaki, A Kuwahara, A Kishino, T Kimura, M Takahashi, M Mandai
    Regenerative Therapy, 2022;19(0):24-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration
    Authors: K Takaya, K Okabe, A Ishigami, Y Imbe, H Kanazawa, S Sakai, N Aramaki-Ha, K Kishi
    Scientific Reports, 2022;12(1):15913.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. EphrinB2-RhoA upregulation attenuates sympathetic hyperinnervation and decreases the incidence of ventricular arrhythmia after myocardial infarction
    Authors: Y Wang, C Shao, L Qi, J Tan, Y Zhao, M Xue, X Li, W Cheng, X Li, J Yin, Y Shi, Y Wang, K Wang, H Hu, S Yan
    Journal of cardiology, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo
    Authors: YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao
    Oxid Med Cell Longev, 2020;2020(0):1404915.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome
    Authors: M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama
    Nat Commun, 2020;11(1):1343.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
    Authors: E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm
    Elife, 2019;8(0):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  8. Inhibition of Ephrin-B2 in brain pericytes decreases cerebral pathological neovascularization in diabetic rats
    Authors: M Coucha, AC Barrett, M Elgebaly, A Ergul, M Abdelsaid
    PLoS ONE, 2019;14(1):e0210523.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells
    Authors: W Shi, Y Wang, J Peng, S Qi, N Vitale, N Kaneda, T Murata, H Luo, J Wu
    J. Biol. Chem., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture Treatment
  10. EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF
    Authors: E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi
    EMBO Rep., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice
    Authors: H Toray, T Hasegawa, N Sakagami, E Tsuchiya, A Kudo, S Zhao, Y Moritani, M Abe, T Yoshida, T Yamamoto, T Yamamoto, K Oda, N Udagawa, PH Luiz de Fr, M Li
    Biomed. Res., 2017;38(2):123-134.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?
    Authors: KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik
    Stem Cell Reports, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016;7(0):12329.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
    Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
    Nat Commun, 2015;6(0):6576.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Proximity Ligation Assay (PLA)
  15. GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
    Authors: Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z
    J Neurosci, 2015;35(13):5409-21.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
    Authors: Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield J
    J Am Soc Nephrol, 2015;24(4):559-72.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
    Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E
    PLoS Genet, 2015;11(11):e1005638.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Biomaterial microenvironments to support the generation of new neurons in the adult brain.
    Authors: Conway A, Schaffer D
    Stem Cells, 2014;32(5):1220-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.
    Authors: Ji Y, Hwang Y, Mood K, Cho H, Lee H, Winterbottom E, Cousin H, Daar I
    Nat Commun, 2014;5(0):3516.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  20. Neuropsin cleaves EphB2 in the amygdala to control anxiety.
    Authors: Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
    Nature, 2011;473(7347):372-5.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate.
    Authors: Williams C, Kim SH, Ni TT, Mitchell L, Ro H, Penn JS, Baldwin SH, Solnica-Krezel L, Zhong TP
    Dev. Biol., 2010;341(1):196-204.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro.
    Authors: He S, Kumar SR, Zhou P, Krasnoperov V, Ryan SJ, Gill PS, Hinton DR
    Invest. Ophthalmol. Vis. Sci., 2010;51(1):543-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
    Authors: Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ
    J. Bone Miner. Res., 2008;23(8):1170-81.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
    Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA
    Biol. Reprod., 2008;79(3):450-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
    Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
    J. Biol. Chem., 2006;281(43):32574-86.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
    Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG
    J. Cell. Sci., 2003;116(0):2461-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Ephrin-B2 Antibody

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Human/Mouse/Rat Ephrin-B2 Antibody
By Anonymous on 03/16/2021
Application: Works well. Unambiguous results!! Sample Tested: Cell culture supernatant,Cell Lysates Species: Human

Human/Mouse/Rat Ephrin-B2 Antibody
By Anonymous on 12/16/2020
Application: ICC/IF Sample Tested: E12.5 mouse embryo fixed in 4% PFA Species: Mouse

Dilution used - 1:200. The staining was done on an E12.5 transverse mouse section fixed overnight with 4% PFA.
Stained using standard IF technique.
Attached picture shows an artery and a vein and it is clearly seen that the artery seen is stained positive whereas the adjacent vein is negative for EphrinB2 (as it should be).