Human/Mouse/Rat Ephrin-B2 Antibody
Human/Mouse/Rat Ephrin-B2 Antibody Summary
Arg27-Ala227
Accession # AAA82934
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Ephrin‑B2 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ephrin-B2 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Ephrin‑B2 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat Ephrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
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Ephrin‑B2 in Rat Hippo-campal Neurons. Ephrin‑B2 was detected in immersion fixed rat hippocampal neurons using 2 µg/mL Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ephrin-B2
Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of murine and human Ephrin-B2 share 98% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).
- Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
- Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
- Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Product Datasheets
Citations for Human/Mouse/Rat Ephrin-B2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Histochemical examination of blood vessels in murine femora with intermittent PTH administration
Authors: H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa
Journal of oral biosciences, 2022;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture
Authors: S Yamasaki, A Kuwahara, A Kishino, T Kimura, M Takahashi, M Mandai
Regenerative Therapy, 2022;19(0):24-34.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration
Authors: K Takaya, K Okabe, A Ishigami, Y Imbe, H Kanazawa, S Sakai, N Aramaki-Ha, K Kishi
Scientific Reports, 2022;12(1):15913.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
EphrinB2-RhoA upregulation attenuates sympathetic hyperinnervation and decreases the incidence of ventricular arrhythmia after myocardial infarction
Authors: Y Wang, C Shao, L Qi, J Tan, Y Zhao, M Xue, X Li, W Cheng, X Li, J Yin, Y Shi, Y Wang, K Wang, H Hu, S Yan
Journal of cardiology, 2021;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo
Authors: YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao
Oxid Med Cell Longev, 2020;2020(0):1404915.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome
Authors: M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama
Nat Commun, 2020;11(1):1343.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development
Authors: E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm
Elife, 2019;8(0):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot -
Inhibition of Ephrin-B2 in brain pericytes decreases cerebral pathological neovascularization in diabetic rats
Authors: M Coucha, AC Barrett, M Elgebaly, A Ergul, M Abdelsaid
PLoS ONE, 2019;14(1):e0210523.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells
Authors: W Shi, Y Wang, J Peng, S Qi, N Vitale, N Kaneda, T Murata, H Luo, J Wu
J. Biol. Chem., 2019;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture Treatment -
EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF
Authors: E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi
EMBO Rep., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice
Authors: H Toray, T Hasegawa, N Sakagami, E Tsuchiya, A Kudo, S Zhao, Y Moritani, M Abe, T Yoshida, T Yamamoto, T Yamamoto, K Oda, N Udagawa, PH Luiz de Fr, M Li
Biomed. Res., 2017;38(2):123-134.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1?
Authors: KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik
Stem Cell Reports, 2017;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
Nat Commun, 2016;7(0):12329.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
EphrinB2 controls vessel pruning through STAT1-JNK3 signalling.
Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G
Nat Commun, 2015;6(0):6576.
Species: Mouse
Sample Types: Cell Lysates
Applications: Proximity Ligation Assay (PLA) -
GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model.
Authors: Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z
J Neurosci, 2015;35(13):5409-21.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury.
Authors: Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield J
J Am Soc Nephrol, 2015;24(4):559-72.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium.
Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E
PLoS Genet, 2015;11(11):e1005638.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Biomaterial microenvironments to support the generation of new neurons in the adult brain.
Authors: Conway A, Schaffer D
Stem Cells, 2014;32(5):1220-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.
Authors: Ji Y, Hwang Y, Mood K, Cho H, Lee H, Winterbottom E, Cousin H, Daar I
Nat Commun, 2014;5(0):3516.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Neuropsin cleaves EphB2 in the amygdala to control anxiety.
Authors: Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R
Nature, 2011;473(7347):372-5.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate.
Authors: Williams C, Kim SH, Ni TT, Mitchell L, Ro H, Penn JS, Baldwin SH, Solnica-Krezel L, Zhong TP
Dev. Biol., 2010;341(1):196-204.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro.
Authors: He S, Kumar SR, Zhou P, Krasnoperov V, Ryan SJ, Gill PS, Hinton DR
Invest. Ophthalmol. Vis. Sci., 2010;51(1):543-52.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts.
Authors: Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ
J. Bone Miner. Res., 2008;23(8):1170-81.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice.
Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA
Biol. Reprod., 2008;79(3):450-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM
J. Biol. Chem., 2006;281(43):32574-86.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.
Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG
J. Cell. Sci., 2003;116(0):2461-70.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Reviews for Human/Mouse/Rat Ephrin-B2 Antibody
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Dilution used - 1:200. The staining was done on an E12.5 transverse mouse section fixed overnight with 4% PFA.
Stained using standard IF technique.
Attached picture shows an artery and a vein and it is clearly seen that the artery seen is stained positive whereas the adjacent vein is negative for EphrinB2 (as it should be).
