Human/Mouse/Rat Ephrin-B2 Antibody

Name: Human/Mouse/Rat Ephrin-B2 Antibody
Brand: R&D Systems

(16 citations)

Datasheet / CoA / SDS

Western Blot

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Flow Cytometry

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Immunocytochemistry

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Product Details

Citations (16)

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Species Reactivity

Human, Mouse, Rat
Specificity

Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Ephrin-B1 is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B2
Arg27-Ala227
Accession # AAA82934
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

1 µg/mL

See below
Flow Cytometry

0.25 µg/10⁶ cells

See below
CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot click image to view larger	Detection of Mouse Ephrin‑B2 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ephrin‑B2 at approximately 40‑45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Flow Cytometry click image to view larger	Detection of Ephrin‑B2 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Immunocytochemistry click image to view larger	Ephrin‑B2 in Rat Hippocampal Neurons. Ephrin‑B2 was detected in immersion fixed rat hippocampal neurons using 2 µg/mL Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-B2

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of murine and human Ephrin-B2 share 98% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

References:
1. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Entrez Gene IDs:

1948 (Human); 13642 (Mouse); 30219 (Zebrafish)

Alternate Names:

EFNB2; ELF-2; EphrinB2; Ephrin-B2; Htk-L; LERK-5; NLERK-1

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species	Applications	Sample Type
Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice Authors: H Toray, T Hasegawa, N Sakagami, E Tsuchiya, A Kudo, S Zhao, Y Moritani, M Abe, T Yoshida, T Yamamoto, T Yamamoto, K Oda, N Udagawa, PH Luiz de Fr, M Li Biomed. Res., 2017;38(2):123-134. Species: Mouse Sample Type: Whole Tissue Application: IHC Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1? Authors: KM Tsang, JS Hyun, KT Cheng, M Vargas, D Mehta, M Ushio-Fuka, L Zou, KV Pajcini, J Rehman, AB Malik Stem Cell Reports, 2017;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 2016;7(0):12329. Species: Mouse Sample Type: Tissue Homogenates Application: WB GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model. Authors: Dong L, Gao F, Wang X, Miao Y, Wang S, Wu Y, Li F, Wu J, Cheng X, Sun X, Yang X, Wang Z J Neurosci, 2015;35(13):5409-21. Species: Rat Sample Type: Tissue Homogenates Application: WB EphrinB2 controls vessel pruning through STAT1-JNK3 signalling. Authors: Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G Nat Commun, 2015;6(0):6576. Species: Mouse Sample Type: Cell Lysates Application: Proximity Ligation Assay Tissue-Specific Effects of Reduced beta-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium. Authors: Feng Y, Sakamoto N, Wu R, Liu J, Wiese A, Green M, Green M, Akyol A, Roy B, Zhai Y, Cho K, Fearon E PLoS Genet, 2015;11(11):e1005638. Species: Mouse Sample Type: Whole Tissue Application: IHC - paraffin EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury. Authors: Kida Y, Ieronimakis N, Schrimpf C, Reyes M, Duffield J J Am Soc Nephrol, 2015;24(4):559-72. Species: Mouse Sample Type: Tissue Homogenates Application: WB EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1. Authors: Ji Y, Hwang Y, Mood K, Cho H, Lee H, Winterbottom E, Cousin H, Daar I Nat Commun, 2014;5(0):3516. Species: Human Sample Type: Cell Lysates Application: IP Biomaterial microenvironments to support the generation of new neurons in the adult brain. Authors: Conway A, Schaffer D Stem Cells, 2014;32(5):1220-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Neuropsin cleaves EphB2 in the amygdala to control anxiety. Authors: Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R Nature, 2011;473(7347):372-5. Species: Mouse Sample Type: Tissue Homogenates Application: WB Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro. Authors: He S, Kumar SR, Zhou P, Krasnoperov V, Ryan SJ, Gill PS, Hinton DR Invest. Ophthalmol. Vis. Sci., 2010;51(1):543-52. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate. Authors: Williams C, Kim SH, Ni TT, Mitchell L, Ro H, Penn JS, Baldwin SH, Solnica-Krezel L, Zhong TP Dev. Biol., 2010;341(1):196-204. Species: Mouse Sample Type: Whole Tissue Application: IHC EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts. Authors: Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ J. Bone Miner. Res., 2008;23(8):1170-81. Species: Rat Sample Type: Cell Lysates Application: WB Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice. Authors: Zhang J, Dong H, Wang B, Zhu S, Croy BA Biol. Reprod., 2008;79(3):450-8. Species: Mouse Sample Type: Whole Cells Application: Flow The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization. Authors: Yang NY, Pasquale EB, Owen LB, Ethell IM J. Biol. Chem., 2006;281(43):32574-86. Species: Mouse Sample Type: Cell Lysates Application: WB Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. Authors: Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG J. Cell. Sci., 2003;116(0):2461-70. Species: Human Sample Type: Cell Lysates Application: WB Expand to show all Citations

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Human/Mouse/Rat Ephrin-B2 Antibody

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